Mitogen-activated protein (MAP) kinase (MAPK) signaling pathway is important in plant immune responses, involved in iron- and reactive oxygen species (ROS)-dependent ferroptotic cell death mediated by Ca. High Ca influx triggered iron-dependent ROS accumulation, lipid peroxidation, and subsequent hypersensitive response (HR) cell death in rice (). Apoplastic Ca chelation by EGTA during avirulent infection altered Ca, ROS, and Fe accumulation, increasing rice susceptibility to infection. By contrast, acibenzolar--methyl (ASM), a plant defense activator, significantly enhanced Ca influx, and HO accumulation, triggering rice ferroptotic cell death during virulent infection. Here, we report a novel role of the MAPK signaling pathway in regulating cytoplasmic Ca increase during ferroptotic cell death in rice immunity, using the knockout mutant rice. The knockout of rice impaired the ROS accumulation, lipid peroxidation, and iron accumulation during avirulent infection. This study has shown that OsMEK2 could positively regulate iron- and ROS-dependent ferroptotic cell death in rice by modulating the expression of , , , and . This modulation indicates a possible mechanism for how OsMEK2 participates in Ca regulation in rice ferroptotic cell death, suggesting its broader role in plant immune responses in response to infection.