Yi Kaige, Ren Yun, Zhang Hui, Lin Baogang, Hao Pengfei, Hua Shuijin
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Institute of Crop, Huzhou Academy of Agricultural Sciences, Huzhou 313000, China.
Antioxidants (Basel). 2024 Aug 22;13(8):1022. doi: 10.3390/antiox13081022.
Rapeseed ( L.) seedlings suffering from inappropriate growth substrate stress will present poor seedling quality. However, the regulatory mechanism for the production and scavenging of reactive oxygen species (ROS) caused by this type of stress remains unclear. In the current study, a split plot experiment design was implemented with two crop growth substrates-a rice growth substrate (RIS) and rapeseed growth substrate (RAS)-as the main plot and two genotypes-a hybrid and an open-pollinated variety (Zheyouza 1510 and Zheyou 51, respectively)-as the sub-plot. The seedling quality was assessed, and the ROS production/scavenging capacity was evaluated. Enzymatic and non-enzymatic systems, including ascorbic acid and glutathione metabolism, and RNA-seq data were analyzed under the two growth substrate treatments. The results revealed that rapeseed seedling quality decreased under RIS, with the plant height, maximum leaf length and width, and aboveground dry matter being reduced by 187.7%, 64.6%, 73.2%, and 63.8% on average, respectively, as compared to RAS. The main type of ROS accumulated in rapeseed plants was hydrogen peroxide, which was 47.8% and 14.1% higher under RIS than under RAS in the two genotypes, respectively. The scavenging of hydrogen peroxide in Zheyouza 1510 was the result of a combination of enzymatic systems, with significantly higher peroxidase (POD) and catalase (CAT) activity as well as glutathione metabolism, with significantly higher reduced glutathione (GSH) content, under RAS, while higher oxidized glutathione (GSSH) was observed under RIS. However, the scavenging of hydrogen peroxide in Zheyou 51 was the result of a combination of elevated oxidized ascorbic acid (DHA) under RIS and higher GSH content under RAS. The identified gene expression levels were in accordance with the observed enzyme expression levels. The results suggest that the cost of substituting RAS with RIS is a reduction in rapeseed seedling quality contributing to excessive ROS production and a reduction in ROS scavenging capacity.
遭受不适当生长基质胁迫的油菜（L.）幼苗会表现出较差的幼苗质量。然而，这种胁迫引起的活性氧（ROS）产生和清除的调控机制仍不清楚。在本研究中，采用裂区试验设计，以两种作物生长基质——水稻生长基质（RIS）和油菜生长基质（RAS）——为主区，两种基因型——一个杂交种和一个开放授粉品种（分别为浙油杂1510和浙油51）——为副区。评估了幼苗质量，并评价了ROS产生/清除能力。在两种生长基质处理下，分析了包括抗坏血酸和谷胱甘肽代谢在内的酶促和非酶促系统以及RNA测序数据。结果表明，在RIS条件下油菜幼苗质量下降，与RAS相比，株高、最大叶长和叶宽以及地上部干物质平均分别降低了187.7%、64.6%、73.2%和63.8%。油菜植株中积累的主要ROS类型是过氧化氢，在两种基因型中，RIS条件下的过氧化氢含量分别比RAS条件下高47.8%和14.1%。浙油杂1510中过氧化氢的清除是酶促系统共同作用的结果，在RAS条件下，过氧化物酶（POD）和过氧化氢酶（CAT）活性显著更高，谷胱甘肽代谢显著更高，还原型谷胱甘肽（GSH）含量显著更高，而在RIS条件下观察到更高的氧化型谷胱甘肽（GSSH）。然而，浙油51中过氧化氢的清除是RIS条件下氧化型抗坏血酸（DHA）升高和RAS条件下GSH含量较高共同作用的结果。鉴定出的基因表达水平与观察到的酶表达水平一致。结果表明，用RIS替代RAS的代价是油菜幼苗质量下降，导致ROS产生过多和ROS清除能力降低。
