Moss Cameron D, Wilson Amber L, Reed Kailee J, Jennings Kaysie J, Kunz Isabelle G Z, Landolt Gabriele A, Metcalf Jessica, Engle Terry E, Coleman Stephen J
Department of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Watchmaker Genomics, Boulder, CO 80301, USA.
Animals (Basel). 2024 Aug 8;14(16):2303. doi: 10.3390/ani14162303.
Previous research demonstrated the distribution of distinct microbial communities in the equine hindgut surrounding the pelvic flexure. The current study evaluated gene expression in epithelial tissues surrounding the pelvic flexure to characterize patterns that might correlate with microbial distribution. Gene expression was determined by analyzing RNA sequence data from the pelvic flexure, the left and right ventral colon, and the left and right dorsal colon. An average of 18,330 genes were expressed across the five tissues sampled. Most of the genes showed some level of expression in all five tissues. Tissue-restricted patterns of expression were also observed. Genes with restricted expression in the left ventral and left dorsal colons have communication, signaling, and regulatory functions that correlate with their known physiology. In contrast, genes expressed exclusively in the pelvic flexure have diverse functions. The ontology of genes differentially expressed between the pelvic flexure and the surrounding tissues was associated with immune functions and signaling processes. Despite being non-significant, these enrichment trends were reinforced by the functions of statistically significant expression differences between tissues of the hindgut. These results provide insight into the physiology of the equine hindgut epithelium that might influence the microbiota and its distribution.
先前的研究表明，在马后肠骨盆曲周围存在不同的微生物群落分布。本研究评估了骨盆曲周围上皮组织中的基因表达，以确定可能与微生物分布相关的模式。通过分析来自骨盆曲、左腹侧结肠和右腹侧结肠以及左背侧结肠和右背侧结肠的RNA序列数据来确定基因表达。在采样的五个组织中平均有18330个基因表达。大多数基因在所有五个组织中都有一定程度的表达。也观察到了组织特异性的表达模式。在左腹侧结肠和左背侧结肠中表达受限的基因具有与它们已知生理学相关的通讯、信号传导和调节功能。相比之下，仅在骨盆曲中表达的基因具有多种功能。骨盆曲与周围组织之间差异表达基因的本体论与免疫功能和信号传导过程相关。尽管不显著，但这些富集趋势通过后肠组织之间具有统计学意义的表达差异的功能得到了加强。这些结果为马后肠上皮的生理学提供了见解，这可能会影响微生物群及其分布。
