Wang Ying, Zuo Kui, Zhang Chi, Miao Dongzhi, Chen Jing, Yang Haiming, Wang Zhiyue
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2024 Aug 10;14(16):2320. doi: 10.3390/ani14162320.
Red light (RL) has been observed to enhance egg production in pigeons, yet the underlying histological characteristics and molecular mechanisms remain less understood. This study included fifty-four pigeons to assess follicular histology, reproductive hormones, and ovarian transcriptomics on the third day of the laying interval under RL and white light (WL). The results showed that the granulosa cell layer was significantly thicker under RL ( < 0.05), whereas the theca cell and connective tissue layers showed no significant differences ( > 0.05). Higher plasma estradiol (E) levels were recorded in the RL group ( < 0.05), as well as follicle stimulating hormone (FSH), although progesterone (P) levels were higher under WL ( < 0.05). Moreover, P concentrations in follicle yolk significantly decreased under RL ( < 0.01), with higher FSH and E levels in F1 yolk and similar increases in SF1 yolk ( < 0.01). Transcriptomic analysis revealed 4991 differentially expressed genes in the pigeon ovary. The protein-protein interaction network highlighted genes like , , , , and as potential contributors to hierarchical follicle selection under RL. This research provides new insights into the molecular basis by which RL may promote hierarchical follicle selection and improve egg production in pigeons.
据观察，红光（RL）可提高鸽子的产蛋量，但其潜在的组织学特征和分子机制仍不太清楚。本研究纳入了54只鸽子，以评估在红光和白光（WL）下产蛋间隔第三天的卵泡组织学、生殖激素和卵巢转录组学。结果显示，红光下颗粒细胞层明显更厚（<0.05），而卵泡膜细胞层和结缔组织层无显著差异（>0.05）。红光组血浆雌二醇（E）水平更高（<0.05），促卵泡激素（FSH）水平也更高，而孕酮（P）水平在白光下更高（<0.05）。此外，红光下卵泡卵黄中的P浓度显著降低（<0.01），F1卵黄中的FSH和E水平更高，SF1卵黄中也有类似升高（<0.01）。转录组分析揭示了鸽子卵巢中有4991个差异表达基因。蛋白质-蛋白质相互作用网络突出显示了如 、 、 、 和 等基因是红光下分级卵泡选择的潜在促成因素。本研究为红光促进鸽子分级卵泡选择和提高产蛋量的分子基础提供了新的见解。
