Peng Qiaoqiao, Lin Junyuan, Wu Yuling, Liao Zengguang, Gu Lihong, Lu Lizhi, Zhang Li
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
Poult Sci. 2025 May 23;104(8):105334. doi: 10.1016/j.psj.2025.105334.
The egg-laying interval reflects the laying performance of pigeons and is affected by the mechanism of ovulation. The Forkhead box O3 (FOXO3) gene is closely associated with follicular development, playing roles in the regulation of follicular granulocyte proliferation, differentiation and periodic ovulation. The objective of this research was to analyze associations of polymorphisms of FOXO3 gene with egg-laying intervals in pigeon. A total of 250 female pigeons of Tianxiang 1 strain were used as experimental materials. Primers were designed according to the sequence of FOXO3 gene in pigeons, and single nucleotide polymorphisms (SNP) of FOXO3 gene were detected by sequencing of PCR products. Twenty SNP sites were found, and 7 of them were located in exons, 1 of them was located in intron, and 12 of them were in the 3' untranslated region (3'UTR) of the gene. Linkage disequilibrium (LD) analysis showed that four blocks were constructed from 20 SNPs. Association analysis showed that 6 SNPs (g.3149417 G > A, g.3149444 C > T, g.3149486 A > G, g.3150038 T > C, g.3151252 G > T and g.3152087 A > G) were significantly associated with egg-laying intervals. Furthermore, we found that the haplotypes of block1 and block4 were significantly correlated with egg-laying intervals. These results suggested that the FOXO3 gene may serve as a candidate gene for laying performance of pigeon.
产蛋间隔反映了鸽子的产蛋性能，并且受排卵机制的影响。叉头框O3（FOXO3）基因与卵泡发育密切相关，在卵泡颗粒细胞增殖、分化及周期性排卵的调控中发挥作用。本研究的目的是分析FOXO3基因多态性与鸽子产蛋间隔的关联。选取250只天翔1系雌性鸽子作为实验材料。根据鸽子FOXO3基因序列设计引物，通过PCR产物测序检测FOXO3基因的单核苷酸多态性（SNP）。共发现20个SNP位点，其中7个位于外显子，1个位于内含子，12个位于基因的3'非翻译区（3'UTR）。连锁不平衡（LD）分析表明，20个SNP构建成4个模块。关联分析表明，6个SNP（g.3149417 G>A、g.3149444 C>T、g.3149486 A>G、g.3150038 T>C、g.3151252 G>T和g.3152087 A>G）与产蛋间隔显著相关。此外，我们发现模块1和模块4的单倍型与产蛋间隔显著相关。这些结果表明，FOXO3基因可能作为鸽子产蛋性能的候选基因。
