Quille Patrick, Higgins Tommy, Neville Enda W, Regan Katy, O'Connell Shane
Shannon Applied Biotechnology Centre, Munster Technological University Kerry, Clash, V92CX88 Tralee, Ireland.
Marigot Researh Centre, Sycamore Court, Clash, V92 N6C8 Tralee, Ireland.
Animals (Basel). 2024 Aug 13;14(16):2333. doi: 10.3390/ani14162333.
The inclusion of rumen buffers in ruminant feeds has gained widespread adoption for the prevention of rumen acidosis, thereby avoiding the negative production and health consequences of low rumen pH and resulting in improved feed efficiency. Benchmarking and quality controlling the performance of rumen buffer materials is of significant interest to feed mills and end-user producers. The aim of this study was to evaluate, develop and optimise a laboratory protocol to consistently and robustly evaluate rumen buffering materials in order to predict their in vivo efficacy. Three different methods were evaluated for determining the buffering potential of carbonate buffer materials: (a) 2 and 8 h static pH, (b) 8 h fixed HCl acid load addition and (c) 3 h acidotic diet simulation using acetic acid. Buffer material, threshold pH, test duration and interactions between all three variables were significant ( < 0.001) in evaluating the performance of the buffer materials. The acidotic diet simulation was found to provide a different ranking of materials to the 8 h fixed HCl acid load methodology. The results highlight the importance of method selection and test parameters for accurately evaluating the potential efficacy of rumen buffer materials.
在反刍动物饲料中添加瘤胃缓冲剂已被广泛采用，用于预防瘤胃酸中毒，从而避免瘤胃低pH值对生产和健康造成的负面影响，并提高饲料效率。对饲料厂和终端用户生产商来说，对瘤胃缓冲剂材料的性能进行基准测试和质量控制具有重大意义。本研究的目的是评估、开发和优化一种实验室方案，以便始终如一地、可靠地评估瘤胃缓冲材料，从而预测其体内功效。评估了三种不同的方法来测定碳酸盐缓冲材料的缓冲潜力：(a) 2小时和8小时静态pH值，(b) 8小时固定添加盐酸酸负荷，以及(c) 使用乙酸进行3小时酸中毒日粮模拟。在评估缓冲材料的性能时，缓冲材料、阈值pH值、测试持续时间以及所有三个变量之间的相互作用均具有显著性（<0.001）。发现酸中毒日粮模拟得出的材料排名与8小时固定盐酸酸负荷方法不同。结果突出了方法选择和测试参数对于准确评估瘤胃缓冲材料潜在功效的重要性。
