Carrillo-Muro Octavio, Rodríguez-Cordero Daniel, Hernández-Briano Pedro, Correa-Aguado Paola Isaira, Medina-Flores Carlos Aurelio, Huerta-López Luis Arturo, Rodríguez-Valdez Francisco Javier, Rivera-Villegas Alejandro, Plascencia Alejandro
Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Zacatecas, General Enrique Estrada 98500, Mexico.
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Sinaloa, Culiacán 80260, Mexico.
Animals (Basel). 2024 Aug 14;14(16):2342. doi: 10.3390/ani14162342.
Enzymic activity, metabolites, and hematological responses for reference intervals (RIs) establish ranges of physiological normality, which are useful for diagnosing diseases and physiological alterations. Within the same species, RIs vary according to age, gender, productive and physiological states, and environmental factors including health management and nutrition. RIs have been extensively studied in dairy calves during a critical stage of life (from birth up to first 90 days of age). A critical stage for feedlot calves is their arrival at the feedlot, but no reports determine RIs for different enzymic activity, metabolites, and hematological responses during their initial period at the feedlot. Consequently, a total of 461 high-risk crossbreed beef calves, received on three different dates, were examined upon arrival at the feedlot. Of these, 320 calves (148.3 ± 1.3 kg body weight) whose "clinical health" was evaluated were included in the study. Blood samples were taken upon arrival and on days 14, 28, 42, and 56 to determine the following parameters: enzymic activity, metabolites, electrolytes, white blood cells, platelets, and red blood cells. Enzymic activity, metabolites, and complete blood count were determined by automated analyzers. The freeware Reference Value Advisor Software was used to calculate the non-parametric values of RIs. This study is the first to establish RIs for different enzymic activity, metabolites, and hematological responses in high-risk newly received calves during their initial period at the feedlot. This information will be useful for veterinary clinical practice and research related to the health and welfare of high-risk newly received calves during their initial period at the feedlot.
用于参考区间（RI）的酶活性、代谢物和血液学反应确定了生理正常范围，这对于诊断疾病和生理改变很有用。在同一物种内，参考区间会因年龄、性别、生产和生理状态以及包括健康管理和营养在内的环境因素而有所不同。参考区间在犊牛生命的关键阶段（从出生到90日龄）已得到广泛研究。饲养场犊牛的一个关键阶段是它们到达饲养场的时候，但尚无报告确定其在饲养场初期不同酶活性、代谢物和血液学反应的参考区间。因此，对分三个不同日期接收的总共461头高危杂交肉牛犊在到达饲养场时进行了检查。其中，对320头评估了“临床健康状况”的犊牛（体重148.3±1.3千克）进行了研究。在到达时以及第14、28、42和56天采集血样，以测定以下参数：酶活性、代谢物、电解质、白细胞、血小板和红细胞。酶活性、代谢物和全血细胞计数由自动分析仪测定。使用免费软件参考值顾问软件计算参考区间的非参数值。本研究首次确定了高危新接收犊牛在饲养场初期不同酶活性、代谢物和血液学反应的参考区间。这些信息将有助于兽医临床实践以及与高危新接收犊牛在饲养场初期的健康和福利相关的研究。
