Yang Guokun, Gu Jianing, Wang Hao, Yang Boya, Feng Shikun, Zhang Yanmin, Zhang Xindang, Chang Xulu, Shao Jianchun, Meng Xiaolin
College of Fisheries, Henan Normal University, Xinxiang 453007, China.
Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, China.
Animals (Basel). 2024 Aug 22;14(16):2443. doi: 10.3390/ani14162443.
Intensive aquaculture of grass carp often leads to decreased immunity and increased disease prevalence, resulting in economic losses. Improving grass carp immunity is therefore a critical strategy for addressing these challenges. Akirin reportedly participates in myogenesis, growth, and immune responses. However, its role in grass carp remains unclear. Herein, we isolated from the spleen of grass carp and analyzed their tissue-specific expression. Akirin expression was detected following treatment with poly (I:C), LPS, and (). The immunological function of the akirin protein was evaluated in head kidney leukocytes (HKLs). The results revealed that the coding sequence (CDS) of is 570 bp, encoding 189 amino acids. There was one predicted nuclear localization signal (NLS) and two predicted α- helix domains. The CDS of is 558 bp, encoding 185 amino acids. There were two predicted NLSs and two predicted α-helix domains. Tissue-specific expression analysis showed that are widely detected in grass carp tissues. was highly detected in the brain, kidneys, heart, spleen, and gonads, while was highly detected in the brain, liver, gonads, kidneys, spleen, and heart. The mRNA levels of were promoted after treatment with poly (I:C), LPS, and . Recombinant akirin proteins were produced in (). , , , , , , and were markedly increased in grass carp HKLs by treatment with the akirin protein. These results suggest that akirins play a role in the immunological regulation of grass carp.
草鱼的集约化养殖常常导致免疫力下降和疾病发生率增加，从而造成经济损失。因此，提高草鱼免疫力是应对这些挑战的关键策略。据报道，Akirin参与肌肉生成、生长和免疫反应。然而，其在草鱼中的作用仍不清楚。在此，我们从草鱼脾脏中分离出（相关物质）并分析它们的组织特异性表达。在用聚肌胞苷酸（poly (I:C)）、脂多糖（LPS）和（此处原文缺失内容）处理后检测到Akirin表达。在头肾白细胞白细胞白细胞白细胞（HKLs）中评估了Akirin蛋白的免疫功能。结果显示，（此处原文缺失具体基因名称）的编码序列（CDS）为570 bp，编码189个氨基酸。有一个预测的核定位信号（NLS）和两个预测的α - 螺旋结构域。（此处原文缺失具体基因名称）的CDS为558 bp，编码185个氨基酸。有两个预测的NLS和两个预测的α - 螺旋结构域。组织特异性表达分析表明，（此处原文缺失具体基因名称）在草鱼组织中广泛检测到。（此处原文缺失具体基因名称）在脑、肾、心脏、脾脏和性腺中高度表达，而（此处原文缺失具体基因名称）在脑、肝脏、性腺、肾、脾脏和心脏中高度表达。在用聚肌胞苷酸（poly (I:C)）、脂多糖（LPS）和（此处原文缺失内容）处理后，（此处原文缺失具体基因名称）的mRNA水平升高。在（此处原文缺失表达系统）中产生了重组Akirin蛋白。用Akirin蛋白处理草鱼HKLs后，（此处原文缺失具体指标）、（此处原文缺失具体指标）、（此处原文缺失具体指标）、（此处原文缺失具体指标）、（此处原文缺失具体指标）、（此处原文缺失具体指标）和（此处原文缺失具体指标）显著增加。这些结果表明Akirin在草鱼的免疫调节中发挥作用。
