Zhang Yunshan, Zhang Xing, Dai Kun, Zhu Min, Liang Zi, Pan Jun, Zhang Ziyao, Xue Renyu, Cao Guangli, Hu Xiaolong, Gong Chengliang
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; Agricultural Biotechnology Research Institute, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou 215123, China.
Int J Biol Macromol. 2022 Jan 1;194:223-232. doi: 10.1016/j.ijbiomac.2021.11.201. Epub 2021 Dec 5.
Bombyx mori cypovirus (BmCPV), a member of the family Reoviridae, is a model of Cypovirus, has a 10 segmented double-stranded RNA genome. However, so far, only one viral small peptide vSP27 with negative regulation on viral infection was identified; the mechanisms underlying host-BmCPV interaction are still unknown. Here, we identified that vSP27 was translated from a BmCPV derived circular RNA (circRNA-vSP27). Subsequently, results showed that vSP27 induced generation of ROS activated the NF-κB signaling pathway, induced the expression of antimicrobial peptides, and suppressed BmCPV infection. On the other hand, we identified a nuclear protein Akirin that could hijack vSP27, positively regulate the NF-κB pathway, and lead to inhibiting the viral infection. Altogether, our data suggested that BmCPV derived circRNA-vSP27 with small peptide translation activity may be employed by the host immunity in defense against the BmCPV infection.
家蚕质型多角体病毒（BmCPV）是呼肠孤病毒科的成员，是质型多角体病毒的一个模型，具有10个节段的双链RNA基因组。然而，到目前为止，仅鉴定出一种对病毒感染具有负调控作用的病毒小肽vSP27；宿主与BmCPV相互作用的潜在机制仍然未知。在此，我们鉴定出vSP27是从BmCPV衍生的环状RNA（circRNA-vSP27）翻译而来。随后，结果表明vSP27诱导活性氧的产生，激活NF-κB信号通路，诱导抗菌肽的表达，并抑制BmCPV感染。另一方面，我们鉴定出一种核蛋白Akirin，它可以劫持vSP27，正向调节NF-κB通路，并导致抑制病毒感染。总之，我们的数据表明，具有小肽翻译活性的BmCPV衍生circRNA-vSP27可能被宿主免疫系统用于抵御BmCPV感染。
