Jantaruk Pornpimon, Teerapo Kittitat, Charoenwutthikun Supattra, Roytrakul Sittiruk, Kunthalert Duangkamol
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Science Park, Pathumthani 12120, Thailand.
Antibiotics (Basel). 2024 Aug 16;13(8):775. doi: 10.3390/antibiotics13080775.
is an opportunistic pathogen in humans and a frequent cause of severe nosocomial infections and fatal infections in immunocompromised individuals. Its ability to form biofilms has been the main driving force behind its resistance to almost all conventional antibiotics, thereby limiting treatment efficacy. In an effort to discover novel therapeutic agents to fight -associated biofilm infections, the truncated analogs of scorpion venom-derived peptide IsCT were synthesized and their anti-biofilm properties were examined. Among the investigated peptides, the IsCT-Δ6-8 peptide evidently showed the most potential anti- biofilm activity and the effect was not due to bacterial growth inhibition. The IsCT-Δ6-8 peptide also exhibited inhibitory activity against the production of pyocyanin, an important virulence factor of . Furthermore, the IsCT-Δ6-8 peptide significantly suppressed the production of inflammatory mediators nitric oxide and interleukin-6 in LPS-induced macrophages. Due to its low cytotoxicity to mammalian cells, the IsCT-Δ6-8 peptide emerges as a promising candidate with significant anti-biofilm and anti-inflammatory properties. These findings highlight its potential application in treating -related biofilm infections.
是人类的一种机会致病菌，是免疫功能低下个体严重医院感染和致命感染的常见原因。其形成生物膜的能力是其对几乎所有传统抗生素产生耐药性的主要驱动力，从而限制了治疗效果。为了发现对抗相关生物膜感染的新型治疗药物，合成了蝎毒衍生肽IsCT的截短类似物，并检测了它们的抗生物膜特性。在所研究的肽中，IsCT-Δ6-8肽显然显示出最具潜力的抗生物膜活性，且这种效果不是由于细菌生长抑制。IsCT-Δ6-8肽还对绿脓菌素（一种的重要毒力因子）的产生表现出抑制活性。此外，IsCT-Δ6-8肽在脂多糖诱导的巨噬细胞中显著抑制炎症介质一氧化氮和白细胞介素-6的产生。由于其对哺乳动物细胞的低细胞毒性，IsCT-Δ6-8肽成为一种具有显著抗生物膜和抗炎特性的有前景的候选物。这些发现突出了其在治疗相关生物膜感染中的潜在应用。
