T淋巴细胞、B淋巴细胞、自然杀伤细胞和单核细胞中端粒长度与不同形式年龄相关性黄斑变性的关联

Association of Telomere Length in T Lymphocytes, B Lymphocytes, NK Cells and Monocytes with Different Forms of Age-Related Macular Degeneration.

作者信息

Khalatyan Anait S, Shishparenok Anastasiya N, Avetisov Konstantin S, Gladilina Yulia A, Blinova Varvara G, Zhdanov Dmitry D

机构信息

Krasnov Research Institute of Eye Diseases, 11A, B, Rossolimo Str., Moscow 119021, Russia.

Laboratory of Medical Biotechnology, Institute of Biomedical Chemistry, 10/8 Pogodinskaya St., Moscow 119121, Russia.

出版信息

Biomedicines. 2024 Aug 19;12(8):1893. doi: 10.3390/biomedicines12081893.

DOI:10.3390/biomedicines12081893

PMID:39200358

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11351114/

Abstract

BACKGROUND

Age plays a primary role in the development of age-related macular degeneration (AMD). Telomere length (TL) is one of the most relevant biomarkers of aging. In our study, we aimed to determine the association of TL with T lymphocytes, B lymphocytes, NK cells or monocytes with different forms of AMD.

METHODS

Our study included 62 patients with AMD: geographic atrophy (GA), neovascular AMD (NVAMD) with and without macular atrophy and 22 healthy controls. Each leukocyte subtype was isolated from peripheral blood by immunomagnetic separation, and the DNA was purified. The TL in the genomic DNA was determined using qPCR by amplifying the telomere region with specific oligonucleotide primers and normalizing to the control gene. Statistical analysis was performed using R version 4.5.1.

RESULTS

We observed a statistically significant increase in TL in the T cells between the control and NVAMD groups but not for the GA group. The B cells and monocytes showed a significant decrease in TL in all AMD groups. The TL in the NK cells did not decrease in any of the AMD groups.

CONCLUSIONS

The TL in the monocytes had the strongest association with AMD. It reflects a person's "telomeric status" and may become a diagnostic hallmark of these degenerative processes.

摘要

背景

年龄在年龄相关性黄斑变性（AMD）的发生发展中起主要作用。端粒长度（TL）是衰老最相关的生物标志物之一。在我们的研究中，我们旨在确定TL与不同形式AMD的T淋巴细胞、B淋巴细胞、自然杀伤（NK）细胞或单核细胞之间的关联。

方法

我们的研究纳入了62例AMD患者，包括地图样萎缩（GA）、有或无黄斑萎缩的新生血管性AMD（NVAMD）以及22名健康对照者。通过免疫磁珠分离法从外周血中分离出每种白细胞亚型，并纯化DNA。使用qPCR通过用特异性寡核苷酸引物扩增端粒区域并与对照基因标准化来测定基因组DNA中的TL。使用R 4.5.1版本进行统计分析。