Isernhagen Lisa, Galuska Christina E, Vernunft Andreas, Galuska Sebastian P
Research Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
Foods. 2024 Aug 7;13(16):2484. doi: 10.3390/foods13162484.
Among other bioactive molecules, milk contains high amounts of sialylated milk oligosaccharides (MOs) that influence numerous processes in the offspring. For instance, sialylated MOs inhibit the invasion of pathogens and positively influence the gut microbiome to support the optimal development of the offspring. For these reasons, sialylated MOs are also used in infant formula as well as food supplements and are potential therapeutic substances for humans and animals. Because of the high interest in sialylated bovine MOs (bMOs), we used several analytical approaches, such as gas and liquid chromatography in combination with mass spectrometry, to investigate in detail the profile of sialylated bMOs in the milk of Holstein Friesian cows during early lactation. Most of the 40 MOs identified in this study were sialylated, and a rapid decrease in all detected sialylated bMOs took place during the first day of lactation. Remarkably, we observed a high variance within the sialylation level during the first two days after calving. Therefore, our results suggest that the content of sialylated MOs might be an additional quality marker for the bioactivity of colostrum and transitional milk to ensure its optimized application for the production of milk replacer and food supplements.
在其他生物活性分子中，牛奶含有大量的唾液酸化乳寡糖（MOs），这些分子会影响后代的众多生理过程。例如，唾液酸化MOs可抑制病原体的侵袭，并对肠道微生物群产生积极影响，以支持后代的最佳发育。基于这些原因，唾液酸化MOs也被用于婴儿配方奶粉以及食品补充剂中，并且是人和动物潜在的治疗物质。由于人们对唾液酸化牛MOs（bMOs）高度关注，我们使用了多种分析方法，如气相色谱和液相色谱结合质谱法，来详细研究荷斯坦弗里生奶牛在泌乳早期牛奶中唾液酸化bMOs的概况。本研究中鉴定出的40种MOs大多是唾液酸化的，并且在泌乳第一天所有检测到唾液酸化bMOs都迅速减少。值得注意的是，我们观察到产犊后的前两天内唾液酸化水平存在很大差异。因此，我们的结果表明，唾液酸化MOs的含量可能是初乳和过渡乳生物活性的一个额外质量指标，以确保其在生产代乳品和食品补充剂中的优化应用。
