Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-356 Poznan, Poland.
Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
Int J Mol Sci. 2024 Aug 9;25(16):8682. doi: 10.3390/ijms25168682.
The aim of the study was to evaluate the diagnostic and prognostic significance of leptin receptor isoforms in adrenal tumors. In a single-center study, 96 patients (19 with adrenal cortical carcinoma and 77 with benign tumors) underwent an adrenalectomy. A total of 14 unaffected adrenal gland tissues from kidney donors were used as controls. Fasting blood samples were collected for laboratory tests, and mRNA expressions of leptin receptor isoforms were assessed by RT-qPCR. The study analyzed correlations between mRNA expressions and clinical data and measured NCI-H295R cell proliferation via a real-time cell analyzer. All adrenal lesions expressed leptin receptor isoforms. Significantly lower LepR1 expression was observed in carcinoma tissues than in adenomas and controls ( = 0.016). Expressions of LepR3&LepR6 were correlated with overall survival ( = 0.036), while LepR2&LepR4 and LepR5 expressions were inversely related to morning serum cortisol levels ( = 0.041). Leptin reduced NCI-H295R cell proliferation ( < 0.0001). The study highlights the diagnostic and prognostic significance of leptin receptor isoforms in adrenal tumors. Specifically, LepR1 may serve as a diagnostic marker for carcinomas, while LepR3&LepR6 have potential use as prognostic markers.
本研究旨在评估瘦素受体异构体在肾上腺肿瘤中的诊断和预后意义。在一项单中心研究中，对 96 例患者（19 例肾上腺皮质癌和 77 例良性肿瘤）进行了肾上腺切除术。总共使用 14 例来自肾供体的无功能肾上腺组织作为对照。采集空腹血样进行实验室检查，并通过 RT-qPCR 评估瘦素受体异构体的 mRNA 表达。该研究分析了 mRNA 表达与临床数据之间的相关性，并通过实时细胞分析仪测量 NCI-H295R 细胞的增殖情况。所有肾上腺病变均表达瘦素受体异构体。与腺瘤和对照组相比，癌组织中 LepR1 的表达明显降低（=0.016）。LepR3 和 LepR6 的表达与总生存时间相关（=0.036），而 LepR2 和 LepR4 以及 LepR5 的表达与早晨血清皮质醇水平呈负相关（=0.041）。瘦素降低了 NCI-H295R 细胞的增殖（<0.0001）。本研究强调了瘦素受体异构体在肾上腺肿瘤中的诊断和预后意义。具体而言，LepR1 可能作为癌的诊断标志物，而 LepR3 和 LepR6 具有作为预后标志物的潜力。
