Department of Removable Prosthodontics, University of Zagreb School of Dental Medicine, Gundulićeva 5, 10000 Zagreb, Croatia.
Laboratory for Epigenomics, Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia.
Int J Mol Sci. 2024 Aug 10;25(16):8730. doi: 10.3390/ijms25168730.
This case-control study investigated single nucleotide polymorphism (SNP) genotypes (CXC motif chemokine ligand 8 (): rs2227306 and rs2227307 and tumor necrosis factor (): rs1800629) in 85 patients with pain-related temporomandibular disorders (TMDp) and 85 controls to explore their associations with TMDp presence, pain intensity (low/high), and the presence of chronic arthralgia/myalgia. TMDp was diagnosed using a validated protocol, and polymorphisms were genotyped from buccal mucosa swabs using TaqMan assays. High pain intensity individuals had an increased risk for carrying minor allele "G" (rs2227307) and "T" (rs2227306) compared to controls (76% vs. 55.3%, = 0.012; 72% vs. 54.1%, = 0.030, respectively). Carriers of the minor allele "G" (rs2227307) were more prevalent in TMDp patients with arthralgia compared to controls (70.30% vs. 55.30%, = 0.037). According to logistic regression, the most important predictors for high pain intensity were minor allele "G" of rs2227307 (OR 2.435, 95% CI 1.123-5.282), increasing age (OR 1.038, 95% CI 1.002-1.075), and female sex (OR 4.592, 95% CI 1.289-16.361). The explored gene polymorphisms were not significant risk factors for TMDp presence. These findings highlight the importance of genetic variations, particularly rs2227307, in understanding the diverse clinical manifestations of temporomandibular disorders.
这项病例对照研究调查了 85 例疼痛相关颞下颌关节紊乱病（TMDp）患者和 85 例对照者的单核苷酸多态性（SNP）基因型（CXC 基序趋化因子配体 8（）：rs2227306 和 rs2227307 以及肿瘤坏死因子（）：rs1800629），以探讨它们与 TMDp 存在、疼痛强度（低/高）以及慢性关节痛/肌痛存在的关系。TMDp 使用经过验证的方案进行诊断，使用 TaqMan 测定法从颊黏膜拭子中对多态性进行基因分型。与对照组相比，高疼痛强度个体携带次要等位基因“G”（rs2227307）和“T”（rs2227306）的风险增加（76%比 55.3%，=0.012；72%比 54.1%，=0.030）。与对照组相比，关节痛 TMDp 患者中携带次要等位基因“G”（rs2227307）的患者更为普遍（70.30%比 55.30%，=0.037）。根据逻辑回归，高疼痛强度的最重要预测因素是 rs2227307 的次要等位基因“G”（OR 2.435，95%CI 1.123-5.282）、年龄增加（OR 1.038，95%CI 1.002-1.075）和女性性别（OR 4.592，95%CI 1.289-16.361）。探索的基因多态性不是 TMDp 存在的显著危险因素。这些发现强调了遗传变异，特别是 rs2227307，在理解颞下颌关节紊乱病不同临床表现方面的重要性。
