Yerliyurt Kaan, Nursal Ayse Feyda, Tekcan Akin, Karakus Nevin, Tumer Mehmet K, Yigit Serbulent
Department of Prosthetic Dentistry, Faculty of Dentistry, Gaziosmanpasa University, Tokat, Turkey.
Department of Medical Genetics, Facultyof Medicine, Hitit University, Corum, Turkey.
J Clin Lab Anal. 2019 Jan;33(1):e22641. doi: 10.1002/jcla.22641. Epub 2018 Aug 20.
Temporomandibular disorders (TMD) are a group of conditions that cause chronic orofacial pain. The tumor necrosis factor β (TNF-β) is a proinflammatory cytokine that is involved in the various aspects of the inflammatory process including organization and maintenance, and in the arrangement of cells at the inflammation site. The purpose of this study was to evaluate the correlation between TNF-β +252A/G (rs909253) variant and susceptibility to TMD in a Turkish cohort.
The study included 104 patients (26 males, 78 females) with TMD and 126 healthy controls (44 males, 82 females). The TNF-β +252A/G variant analysis was based on Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
There was no deviation from HWA for TNF-β +252A/G variant in patient and control groups. There was significant difference in genotype and allele frequencies between patient group and control group in terms of TNF-β +252A/G variant, respectively (P = 0.010, 0.015). A significant increase in the TNF-β +252 AG genotype and G allele frequencies were observed in TMD patients compared to healthy controls. The individuals with GG genotype and G allele had an increased risk of developing TMD. A statistically significant association was observed when the patients were compared with the controls according to AA genotype vs AG+GG genotypes (P = 0.002, OR: 2.23, 95% CI:1.31-3.82). TNF-β +252A/G genotype distribution was associated with chewing problems (P = 0.046).
In conclusion, our results provided evidence that TNF-β +252A/G variant may contribute to TMD development in a Turkish cohort. Further studies are needed to confirm this observation.
颞下颌关节紊乱病（TMD）是一组导致慢性口面部疼痛的病症。肿瘤坏死因子β（TNF-β）是一种促炎细胞因子，参与炎症过程的各个方面，包括组织和维持，以及炎症部位细胞的排列。本研究的目的是评估土耳其人群中TNF-β +252A/G（rs909253）变异与TMD易感性之间的相关性。
该研究纳入了104例TMD患者（26例男性，78例女性）和126名健康对照者（44例男性，82例女性）。TNF-β +252A/G变异分析基于聚合酶链反应-限制性片段长度多态性（PCR-RFLP）。
患者组和对照组中TNF-β +252A/G变异均未偏离哈迪-温伯格平衡。在TNF-β +252A/G变异方面，患者组和对照组的基因型和等位基因频率分别存在显著差异（P = 0.010，0.015）。与健康对照相比，TMD患者中TNF-β +252 AG基因型和G等位基因频率显著增加。具有GG基因型和G等位基因的个体患TMD的风险增加。根据AA基因型与AG+GG基因型将患者与对照进行比较时，观察到具有统计学意义的关联（P = 0.002，OR：2.23，95%CI：1.31 - 3.82）。TNF-β +252A/G基因型分布与咀嚼问题相关（P = 0.046）。
总之，我们的结果提供了证据表明TNF-β +252A/G变异可能在土耳其人群中促成TMD的发生。需要进一步的研究来证实这一观察结果。
