一株人源粪便大肠杆菌菌株对小鼠结肠黏液的黏附作用。
Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus.
作者信息
Cohen P S, Arruda J C, Williams T J, Laux D C
出版信息
Infect Immun. 1985 Apr;48(1):139-45. doi: 10.1128/iai.48.1.139-145.1985.
Abstract
Escherichia coli F-18 isolated from the feces of a healthy human is an excellent colonizer of the CD-1 mouse colon. In the present investigation, adhesion of E. coli F-18 to CD-1 mouse colonic mucus and bovine serum albumin (BSA), immobilized on polystyrene, was studied. Adhesion of E. coli F-18 to mucus was two- to sixfold greater than to either BSA or polystyrene. E. coli F-18 lipopolysaccharide specifically blocked adhesion of E. coli F-18 to mucus and mimicked adhesion of E. coli F-18 to mucus, BSA, and polystyrene. Purified capsule also blocked adhesion of E. coli F-18 to mucus, but this inhibition was found to be entirely nonspecific. The specific E. coli F-18 receptor in mucus appeared to be a glycoprotein, containing sugars normally found in mucins and having a maximum molecular weight of between 1.25 X 10(5) and 2.5 X 10(5).
摘要
从健康人体粪便中分离出的大肠杆菌F-18是CD-1小鼠结肠的优良定植菌。在本研究中，研究了大肠杆菌F-18对固定在聚苯乙烯上的CD-1小鼠结肠黏液和牛血清白蛋白（BSA）的黏附情况。大肠杆菌F-18对黏液的黏附比对BSA或聚苯乙烯的黏附大两到六倍。大肠杆菌F-18脂多糖特异性地阻断了大肠杆菌F-18对黏液的黏附，并模拟了大肠杆菌F-18对黏液、BSA和聚苯乙烯的黏附。纯化的荚膜也阻断了大肠杆菌F-18对黏液的黏附，但发现这种抑制完全是非特异性的。黏液中大肠杆菌F-18的特异性受体似乎是一种糖蛋白，含有通常在黏蛋白中发现的糖类，最大分子量在1.25×10⁵至2.5×10⁵之间。
相似文献
1
Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus.一株人源粪便大肠杆菌菌株对小鼠结肠黏液的黏附作用。
Infect Immun. 1985 Apr;48(1):139-45. doi: 10.1128/iai.48.1.139-145.1985.
2
Colonization of the streptomycin-treated mouse large intestine by a human fecal Escherichia coli strain: role of adhesion to mucosal receptors.一株人粪便大肠杆菌菌株在经链霉素处理的小鼠大肠中的定殖：对黏膜受体黏附的作用
Infect Immun. 1988 May;56(5):1036-43. doi: 10.1128/iai.56.5.1036-1043.1988.
3
Type 1 pili are not necessary for colonization of the streptomycin-treated mouse large intestine by type 1-piliated Escherichia coli F-18 and E. coli K-12.1型菌毛对于1型菌毛化的大肠杆菌F-18和大肠杆菌K-12在经链霉素处理的小鼠大肠中定殖并非必需。
Infect Immun. 1989 Oct;57(10):3022-9. doi: 10.1128/iai.57.10.3022-3029.1989.
4
Relationship between the mouse colonizing ability of a human fecal Escherichia coli strain and its ability to bind a specific mouse colonic mucous gel protein.一株人粪便大肠杆菌菌株的小鼠定殖能力与其结合特定小鼠结肠黏液凝胶蛋白的能力之间的关系
Infect Immun. 1983 Apr;40(1):62-9. doi: 10.1128/iai.40.1.62-69.1983.
5
Colonization of the streptomycin-treated mouse large intestine by a human fecal Escherichia coli strain: role of growth in mucus.一株人粪便大肠杆菌菌株在经链霉素处理的小鼠大肠中的定殖：在黏液中生长的作用
Infect Immun. 1988 May;56(5):1030-5. doi: 10.1128/iai.56.5.1030-1035.1988.
6
Identification and characterization of mouse small intestine mucosal receptors for Escherichia coli K-12(K88ab).小鼠小肠黏膜对大肠杆菌K-12（K88ab）受体的鉴定与特性分析
Infect Immun. 1986 Apr;52(1):18-25. doi: 10.1128/iai.52.1.18-25.1986.
7
Neither motility nor chemotaxis plays a role in the ability of Escherichia coli F-18 to colonize the streptomycin-treated mouse large intestine.运动性和趋化性在大肠杆菌F-18定殖于经链霉素处理的小鼠大肠的能力中均不起作用。
Infect Immun. 1990 Sep;58(9):2957-61. doi: 10.1128/iai.58.9.2957-2961.1990.
8
Mucin isolated from rabbit colon inhibits in vitro binding of Escherichia coli RDEC-1.从兔结肠分离出的粘蛋白可抑制大肠杆菌RDEC-1的体外结合。
Infect Immun. 1991 Mar;59(3):1015-23. doi: 10.1128/iai.59.3.1015-1023.1991.
9
Escherichia coli F-18 phase locked 'on' for expression of type 1 fimbriae is a poor colonizer of the streptomycin-treated mouse large intestine.用于表达1型菌毛的大肠杆菌F-18在链霉素处理的小鼠大肠中是一种定植能力较差的菌株。
Microb Pathog. 1993 Jan;14(1):33-43. doi: 10.1006/mpat.1993.1004.
10
In vivo colonization of the mouse large intestine and in vitro penetration of intestinal mucus by an avirulent smooth strain of Salmonella typhimurium and its lipopolysaccharide-deficient mutant.鼠伤寒沙门氏菌无毒光滑菌株及其脂多糖缺陷型突变体在小鼠大肠内的体内定殖和在体外对肠黏液的穿透。
Infect Immun. 1987 Dec;55(12):2884-90. doi: 10.1128/iai.55.12.2884-2890.1987.
引用本文的文献
1
Morphogenesis of bacterial cables in polymeric environments.聚合物环境中细菌电缆的形态发生
Sci Adv. 2025 Jan 17;11(3):eadq7797. doi: 10.1126/sciadv.adq7797.
2
Glycan:glycan interactions: High affinity biomolecular interactions that can mediate binding of pathogenic bacteria to host cells.聚糖：聚糖相互作用：可介导病原菌与宿主细胞结合的高亲和力生物分子相互作用。
Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):E7266-75. doi: 10.1073/pnas.1421082112. Epub 2015 Dec 16.
3
Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells.产肠毒素大肠杆菌分泌一种高度保守的降解黏蛋白的金属蛋白酶，以有效黏附肠道上皮细胞。
Infect Immun. 2014 Feb;82(2):509-21. doi: 10.1128/IAI.01106-13. Epub 2013 Nov 18.
4
Yeast colonizing the intestine of rainbow trout (Salmo gairdneri) and turbot (Scophtalmus maximus).肠道中定植的酵母（虹鳟和大菱鲆）。
Microb Ecol. 1995 Nov;30(3):321-34. doi: 10.1007/BF00171938.
5
Utility of in vivo transcription profiling for identifying Pseudomonas aeruginosa genes needed for gastrointestinal colonization and dissemination.体内转录谱分析鉴定铜绿假单胞菌胃肠道定植和传播所需基因的效用。
PLoS One. 2010 Dec 10;5(12):e15131. doi: 10.1371/journal.pone.0015131.
6
Energy taxis drives Campylobacter jejuni toward the most favorable conditions for growth.能量趋化驱使空肠弯曲菌朝着最适宜生长的条件移动。
Appl Environ Microbiol. 2009 Aug;75(16):5308-14. doi: 10.1128/AEM.00287-09. Epub 2009 Jun 19.
7
Binding of Actinobacillus pleuropneumoniae to phosphatidylethanolamine.胸膜肺炎放线杆菌与磷脂酰乙醇胺的结合。
Infect Immun. 2003 Aug;71(8):4657-63. doi: 10.1128/IAI.71.8.4657-4663.2003.
8
Construction and characterization of genetically defined aro omp mutants of enterotoxigenic Escherichia coli and preliminary studies of safety and immunogenicity in humans.产肠毒素大肠杆菌基因定义的aro omp突变体的构建与特性分析以及在人体中的安全性和免疫原性初步研究。
Infect Immun. 2001 Aug;69(8):4969-79. doi: 10.1128/IAI.69.8.4969-4979.2001.
9
Biofilm formation in a hydrodynamic environment by novel fimh variants and ramifications for virulence.新型fimh变体在流体动力学环境中的生物膜形成及其对毒力的影响。
Infect Immun. 2001 Mar;69(3):1322-8. doi: 10.1128/IAI.69.3.1322-1328.2001.
10
Intestinal mucins: the binding sites for Salmonella typhimurium.肠道黏蛋白：鼠伤寒沙门氏菌的结合位点。
Mol Cell Biochem. 2000 Jan;204(1-2):107-17. doi: 10.1023/a:1007015312036.
本文引用的文献
1
R-Plasmid Transfer to and from Escherichia coli Strains Isolated from Human Fecal Samples.人粪便中分离的大肠杆菌菌株的 R 质粒转移到和从大肠杆菌菌株的转移
Appl Environ Microbiol. 1981 Apr;41(4):959-66. doi: 10.1128/aem.41.4.959-966.1981.
2
New color reactions for determination of sugars in polysaccharides.用于测定多糖中糖类的新显色反应。
Methods Biochem Anal. 1955;2:313-58. doi: 10.1002/9780470110188.ch11.
3
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.唾液酸的定量测定。II. 比色间苯二酚 - 盐酸法。
Biochim Biophys Acta. 1957 Jun;24(3):604-11. doi: 10.1016/0006-3002(57)90254-8.
4
Role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vitro studies.趋化作用在运动性细菌与肠黏膜结合中的作用：体外研究
Infect Immun. 1981 Oct;34(1):241-9. doi: 10.1128/iai.34.1.241-249.1981.
5
Adherence of Shigella flexneri to guinea pig intestinal cells is mediated by a mucosal adhesion.弗氏志贺菌对豚鼠肠道细胞的黏附是由一种黏膜黏附素介导的。
Infect Immun. 1982 Mar;35(3):1110-8. doi: 10.1128/iai.35.3.1110-1118.1982.
6
Modified method for studying bacterial adhesion to isolated uroepithelial cells and uromucoid.研究细菌对分离的尿道上皮细胞和尿类粘蛋白粘附作用的改良方法。
Infect Immun. 1981 Oct;34(1):256-61. doi: 10.1128/iai.34.1.256-261.1981.
7
Mechanisms of association of bacteria with mucosal surfaces.细菌与黏膜表面结合的机制。
Ciba Found Symp. 1981;80:36-55. doi: 10.1002/9780470720639.ch4.
8
Adherence of Pseudomonas aeruginosa to human tracheobronchial mucin.铜绿假单胞菌对人气管支气管黏蛋白的黏附作用。
Infect Immun. 1984 Jul;45(1):197-202. doi: 10.1128/iai.45.1.197-202.1984.
9
Altered colonizing ability for mouse large intestine of a surface mutant of a human faecal isolate of Escherichia coli.人粪便分离的大肠杆菌表面突变体对小鼠大肠的定殖能力改变。
J Gen Microbiol. 1983 May;129(5):1549-58. doi: 10.1099/00221287-129-5-1549.
10
Relationship between the mouse colonizing ability of a human fecal Escherichia coli strain and its ability to bind a specific mouse colonic mucous gel protein.一株人粪便大肠杆菌菌株的小鼠定殖能力与其结合特定小鼠结肠黏液凝胶蛋白的能力之间的关系
Infect Immun. 1983 Apr;40(1):62-9. doi: 10.1128/iai.40.1.62-69.1983.
Zotero 插件