HUN-REN Veterinary Medical Research Institute, 1143 Budapest, Hungary.
National Food Chain Safety Office, 1095 Budapest, Hungary.
Int J Mol Sci. 2024 Aug 13;25(16):8820. doi: 10.3390/ijms25168820.
Rats are particularly important from an epidemiological point of view, because they are regarded as reservoirs for diverse zoonotic pathogens including enteric bacteria. This study is the first to report the emergence of serovar Ohio in brown rats () and food-producing animals in Hungary. We first reveal the genomic diversity of the strains and their phylogenomic relationships in the context of the international collection of . Ohio genomes. This pathogen was detected in 4.3% (4/92) of rats, captured from multiple sites in Hungary. A whole-genome-based genotype comparison of . Ohio, Infantis, Enteritidis, and Typhimurium strains showed that 76.4% (117/153) of the virulence and antimicrobial resistance genes were conserved among these serovars, and none of the genes were specific to . Ohio. All . Ohio strains lacked virulence and resistance plasmids. The cgMLST phylogenomic comparison highlighted a close genetic relationship between rat and poultry strains of . Ohio from Hungary. These strains clustered together with the international . Ohio genomes from aquatic environments. Overall, this study contributes to our understanding of the epidemiology of spp. in brown rats and highlights the importance of monitoring to minimize the public health risk of rodent populations. However, further research is needed to understand the route of infection and evolution of this serovar.
从流行病学的角度来看，大鼠尤为重要，因为它们被认为是多种人畜共患病病原体的宿主，包括肠道细菌。本研究首次报道了肠球菌血清型俄亥俄在匈牙利的褐家鼠（）和生产性动物中出现。我们首次揭示了这些菌株的基因组多样性及其在国际肠球菌属基因组集合中的系统发育关系。在匈牙利多个地点捕获的 92 只大鼠中，有 4.3%（4/92）检测到该病原体。对俄亥俄、婴儿、肠炎和鼠伤寒血清型的基于全基因组的基因型比较表明，这些血清型之间 76.4%（117/153）的毒力和抗微生物耐药基因是保守的，并且没有任何基因是俄亥俄血清型所特有的。所有俄亥俄菌株均缺乏毒力和耐药质粒。cgMLST 系统发育比较突出了来自匈牙利的大鼠和家禽俄亥俄菌株之间的密切遗传关系。这些菌株与水生环境中的国际俄亥俄基因组聚集在一起。总的来说，本研究有助于我们了解褐家鼠中 spp. 的流行病学，并强调了监测的重要性，以最大限度地降低啮齿动物种群的公共卫生风险。然而，需要进一步研究来了解该血清型的感染途径和进化。
