Department of Medical Microbiology, Semmelweis University, 1089 Budapest, Hungary.
Department of Vascular and Endovascular Surgery, Heart and Vascular Center, Semmelweis University, 1122 Budapest, Hungary.
Int J Mol Sci. 2024 Aug 14;25(16):8844. doi: 10.3390/ijms25168844.
Previous research confirmed gut dysbiosis and translocation of selected intestinal bacteria into the vessel wall in abdominal aortic aneurysm patients. We studied the stool, blood, thrombus and aneurysm microbiomes of 21 abdominal aortic aneurysm patients using 16S rRNA sequencing. Our goals were to determine: 1. whether the microbiome characteristic of an aneurysm differs from that of a healthy vessel, 2. whether bacteria detectable in the aneurysm are translocated from the gut through the bloodstream, 3. whether the enzymatic activity of the aneurysm microbiome can contribute to the destruction of the vessel wall. The abundance of , , , and in the aneurysm samples was significantly higher than that in the microbiome of healthy vessels, but only a part of these bacteria can come from the intestine via the blood. Environmental bacteria due to the oral cavity or skin penetration route, such as , , , , were present in the thrombus and aneurysm with a significantly higher abundance compared to the blood. Among the enzymes of the microbiome associated with the healthy vessel wall, Iron-chelate-transporting ATPase and Polar-amino-acid-transporting ATPase have protective effects. In addition, bacterial Peptidylprolyl isomerase activity found in the aneurysm has an aggravating effect on the formation of aneurysm.
先前的研究证实，腹主动脉瘤患者存在肠道菌群失调和特定肠道细菌易位至血管壁的现象。我们通过 16S rRNA 测序技术对 21 例腹主动脉瘤患者的粪便、血液、血栓和瘤体微生物组进行了研究。我们的目标是确定：1. 动脉瘤的微生物组特征是否与健康血管不同，2. 可在动脉瘤中检测到的细菌是否通过血液从肠道易位而来，3. 动脉瘤微生物组的酶活性是否有助于破坏血管壁。在动脉瘤样本中， 、 、 、 和 的丰度明显高于健康血管的微生物组，但这些细菌只有一部分可以通过血液从肠道来。由于口腔或皮肤穿透途径而进入的环境细菌，如 、 、 、 ，在血栓和动脉瘤中的丰度明显高于血液。与健康血管壁相关的微生物组中的酶中，铁螯合转运 ATP 酶和极性氨基酸转运 ATP 酶具有保护作用。此外，在动脉瘤中发现的细菌肽基脯氨酰异构酶活性对动脉瘤的形成有加重作用。
