Department of Molecular Oral Medicine and Maxillofacial Surgery, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima 734-8553, Japan.
Department of Oral and Maxillofacial Surgery, Hiroshima University Hospital, Hiroshima 734-8553, Japan.
Int J Mol Sci. 2024 May 31;25(11):6077. doi: 10.3390/ijms25116077.
The impact of gut and oral microbiota on the clinical outcomes of patients with oral squamous cell carcinoma (OSCC) is unknown. We compared the bacterial composition of dental plaque and feces between patients with OSCC and healthy controls (HCs). Fecal and dental plaque samples were collected from 7 HCs and 18 patients with OSCC before treatment initiation. Terminal restriction fragment-length polymorphism analysis of 16S rRNA genes was performed. Differences in bacterial diversity between the HC and OSCC groups were examined. We compared the occupancy of each bacterial species in samples taken from patients with OSCC and HCs and analyzed the correlation between PD-L1 expression in the tumor specimens and the occupancy of each bacterial species. The gut and oral microbiota of patients with OSCC were more varied than those of HCs. and were significantly more abundant in patients with OSCC than in HCs. The abundance of subcluster XIVa in the gut microbiota of the PD-L1-positive group was significantly greater than that in the PD-L1-negative group. The oral and gut microbiomes of patients with OSCC were in a state of dysbiosis. Our results suggest the possibility of new cancer therapies targeting these disease-specific microbiomes using probiotics and synbiotics.
口腔鳞状细胞癌（OSCC）患者的肠道和口腔微生物群对临床结局的影响尚不清楚。我们比较了 OSCC 患者和健康对照者（HCs）的牙菌斑和粪便中的细菌组成。在开始治疗前，从 7 名 HCs 和 18 名 OSCC 患者中收集粪便和牙菌斑样本。对 16S rRNA 基因进行末端限制性片段长度多态性分析。检查 HC 和 OSCC 组之间细菌多样性的差异。我们比较了来自 OSCC 患者和 HCs 的样本中每种细菌的定植情况，并分析了肿瘤标本中 PD-L1 表达与每种细菌定植之间的相关性。OSCC 患者的肠道和口腔微生物群比 HCs 的更具多样性。和在 OSCC 患者中明显比 HCs 更丰富。PD-L1 阳性组肠道微生物群中 XIVa 亚群的丰度明显高于 PD-L1 阴性组。OSCC 患者的口腔和肠道微生物群处于生态失调状态。我们的研究结果表明，使用益生菌和合生菌靶向这些疾病特异性微生物群的新癌症疗法具有可能性。
