State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650032, China.
School of Life Science, Yunnan University, Kunming 650032, China.
Int J Mol Sci. 2024 Aug 19;25(16):9013. doi: 10.3390/ijms25169013.
Limited exploration in fungal mitochondrial genetics has uncovered diverse inheritance modes. The mitochondrial genomes are inherited uniparentally in the majority of sexual eukaryotes, our discovery of persistent mitochondrial heterogeneity within the natural population of the basidiomycete fungus represents a significant advance in understanding mitochondrial inheritance and evolution in eukaryotes. Here, we present a comprehensive analysis by sequencing and assembling the complete mitogenomes of 40 samples exhibiting diverse heterogeneity patterns from various geographical origins. Additionally, we identified heterogeneous variants in the gene, which, similar to , displayed variability across multiple copies. Notably, our study reveals a distinct prevalence of introns and homing endonucleases in these heterogeneous genes. Furthermore, we detected potential instances of horizontal gene transfer involving homing endonucleases. Population genomic analyses underscore regional variations in mitochondrial genome composition among natural samples exhibiting heterogeneity. Thus, polymorphisms in heterogeneous genes, introns, and homing endonucleases significantly influence mitochondrial structure, structural variation, and evolutionary dynamics in this species. This study contributes valuable insights into mitochondrial genome architecture, population dynamics, and the evolutionary implications of mitochondrial heterogeneity in sexual eukaryotes.
真菌线粒体遗传学的有限探索揭示了多样化的遗传模式。在大多数有性真核生物中，线粒体基因组是单系遗传的，我们在担子菌真菌的自然种群中发现了线粒体异质性的持续存在，这代表了在理解有性真核生物中线粒体遗传和进化方面的重大进展。在这里，我们通过对来自不同地理来源的 40 个表现出不同异质模式的样本进行测序和组装，全面分析了完整的线粒体基因组。此外，我们还鉴定了基因中的异质变体，该变体与类似，在多个拷贝中表现出可变性。值得注意的是，我们的研究揭示了这些异质基因中内含子和归巢内切酶的明显普遍性。此外，我们检测到涉及归巢内切酶的潜在水平基因转移实例。群体基因组分析强调了自然样本中表现出异质性的线粒体基因组组成在区域上的差异。因此，异质基因、内含子和归巢内切酶中的多态性显著影响了该物种的线粒体结构、结构变异和进化动态。本研究为线粒体基因组结构、种群动态以及有性真核生物中线粒体异质性的进化意义提供了有价值的见解。
