School of Forestry, Northeast Forestry University, Harbin 150040, China.
Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2024 Aug 21;25(16):9070. doi: 10.3390/ijms25169070.
Insects rely on olfaction for mating, finding oviposition sites, and locating hosts. is a serious pest that severely damages forests. Differential expression analysis of olfactory-related genes between males and females is the basis for elucidating the functions of olfactory-related proteins in . In this study, Illumina HiSeqTM 4000 high-throughput sequencing technology was used to perform transcriptome sequencing of the antennal tissues of adult male and female . Functional annotation was conducted using the NR, Swiss-Prot, KOG, KEGG, and GO databases, and the results showed that the antennal transcriptome of adult contained 50,158 unigenes. Differential expression analysis identified 3923 genes that were significantly differentially expressed between male and female antennae. A total of 221 olfactory-related genes were annotated, and 96 sex-biased genes were identified, including 13 odorant receptors (ORs), 48 odorant binding proteins (OBPs), 7 chemosensory proteins (CSPs), 10 ionotropic receptors (IRs), 10 sensory neuron membrane proteins (SNMPs), 2 gustatory receptors (GRs), and 6 odorant-degrading enzymes (ODEs), indicating that there were differences in olfaction between male and female . Quantitative real-time PCR was used to verify the expression levels of 21 putative general odorant receptor genes in male and female antennae. and showed female-biased expression; , and showed male-biased expression. The results were consistent with the transcriptome differential analysis. The screening of male-biased odorant receptor genes might provide a theoretical basis for the functional characterization of odorant receptors for recognizing sex pheromones in .
昆虫依靠嗅觉进行交配、寻找产卵地和定位宿主。 是一种严重的害虫，会严重破坏森林。雄性和雌性昆虫嗅觉相关基因的差异表达分析是阐明嗅觉相关蛋白在 中的功能的基础。在这项研究中，使用 Illumina HiSeqTM 4000 高通量测序技术对成年雄性和雌性 的触角组织进行转录组测序。使用 NR、Swiss-Prot、KOG、KEGG 和 GO 数据库进行功能注释，结果表明，成年 的触角转录组包含 50158 个 unigenes。差异表达分析确定了 3923 个在雌雄触角之间表达差异显著的基因。共注释了 221 个嗅觉相关基因，鉴定出 96 个性别偏向基因，包括 13 个气味受体 (ORs)、48 个气味结合蛋白 (OBPs)、7 个化学感受蛋白 (CSPs)、10 个离子型受体 (IRs)、10 个感觉神经元膜蛋白 (SNMPs)、2 个味觉受体 (GRs)和 6 个气味降解酶 (ODEs)，表明雌雄之间的嗅觉存在差异。定量实时 PCR 用于验证 21 个假定的雄性和雌性触角通用气味受体基因的表达水平。 和 表现出雌性偏向表达； 、 和 表现出雄性偏向表达。结果与转录组差异分析一致。雄性偏向气味受体基因的筛选可能为识别 性信息素的气味受体的功能表征提供理论依据。
