, , and DNA Methylation Strongly Estimate Indonesian Adolescents.

Recently, there has been a growing interest in using DNA methylation analysis for age estimation. Despite this growing interest, there is a scarcity of research on the potential of DNA methylation as a biomarker for age estimation in Indonesia. This study aims to investigate the applicability of , , and genes for forensic identification in the 11-20 age group among Indonesians. This research utilizes 43 archived blood samples from healthy individuals who underwent blood tests at the Gatot Soebroto Army Hospital (RSPAD) in Central Jakarta, Indonesia. The methylation-specific PCR (MSP) technique assessed the DNA methylation level. The key findings of this study include (1) a strong positive correlation between methylation levels in the gene and age; (2) a strong negative correlation between methylation levels in and genes with age; (3) the development of three linear regression formulas for age prediction; and (4) mean absolute error (MAE) values derived from this research, which are ±0.48 for gene regression formula, ±0.58 for gene regression formula, and ±0.72 for gene regression formula. In summary, this study explores the potential of DNA methylation analysis for age estimation in Indonesia, focusing on , , and genes in the 11-20 age group. The findings underscore the applicability of DNA methylation analysis in forensic identification and age estimation, paving the way for future research in this field.