Stojiljković Stanimir, Gavrilović Ljubica, Pejić Snežana, Pajović Snežana B, Macura Marija, Nikolić Dragan, Bubanj Saša, Stojiljković Vesna
Faculty of Sport and Physical Education, University of Belgrade, 11000 Belgrade, Serbia.
Department of Molecular Biology and Endocrinology, "Vinča" Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.
Life (Basel). 2024 Jul 24;14(8):921. doi: 10.3390/life14080921.
(1) Background: Physical activity may cause an imbalance in the major functions of the human body. This study aimed to investigate the effects of endurance running training on the parameters of the antioxidant defense system (SOD, CAT, GPx, GR, GSH), LPO (malondialdehyde, MDA), and stress hormones (A, NA) in young healthy, previously untrained men. (2) Methods: The training program was as follows: 8 weeks of running, three times per week; the duration of a single session was 30-70 min, the intensity was twice a week in the so-called extensive endurance zone, and once a week in the anaerobic threshold zone. Blood samples were collected from the subjects, before and after the running program. (3) Results: The training program resulted in a significant increase in maximal oxygen consumption ( < 0.001). The activities of SOD, GPx, and GR also increased significantly ( < 0.05, < 0.01, and < 0.05, respectively), while CAT activity and GSH and MDA concentrations remained unchanged. The concentration of A decreased ( < 0.05), while the NA concentration increased significantly ( < 0.05). SOD, GPx, GR, and NA positively correlated with VOmax ( < 0.05, < 0.001, < 0.01, < 0.05, respectively), while a negative correlation was detected between A and VOmax ( < 0.05). (4) Conclusions: These results indicate that there is no persistent oxidative stress in response to the applied 8-week running program, probably due to exercise-induced protective alterations in the antioxidant defense system. Furthermore, adaptations occurred at the hormonal level, making the organism more ready for a new challenge.
(1) 背景：体育活动可能会导致人体主要功能失衡。本研究旨在调查耐力跑训练对年轻健康、此前未经训练的男性抗氧化防御系统参数（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽）、脂质过氧化（丙二醛、MDA）以及应激激素（肾上腺素、去甲肾上腺素）的影响。(2) 方法：训练计划如下：为期8周的跑步训练，每周3次；单次训练时长为30 - 70分钟，强度为每周两次处于所谓的广泛耐力区间，每周一次处于无氧阈值区间。在跑步训练计划前后采集受试者的血样。(3) 结果：训练计划导致最大摄氧量显著增加（<0.001）。超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性也显著增加（分别为<0.05、<0.01和<0.05），而过氧化氢酶活性以及谷胱甘肽和丙二醛浓度保持不变。肾上腺素浓度降低（<0.05），而去甲肾上腺素浓度显著增加（<0.05）。超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和去甲肾上腺素与最大摄氧量呈正相关（分别为<0.05、<0.001、<0.01、<0.05），而肾上腺素与最大摄氧量之间存在负相关（<0.05）。(4) 结论：这些结果表明，针对所应用的为期8周的跑步计划，未出现持续性氧化应激，这可能是由于运动诱导抗氧化防御系统发生保护性改变所致。此外，在激素水平出现了适应性变化，使机体更能应对新的挑战。
