Shanxi Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Toxicology of Ministry of Public Security, School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, China.
Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
Molecules. 2024 Aug 16;29(16):3881. doi: 10.3390/molecules29163881.
The widespread use of per- and polyfluoroalkyl substances (PFASs) with different physico-chemical properties poses a great threat to the environment and human health. Simultaneous detection of different classes of PFASs is a difficult task, especially for rapid analysis of polluted water samples in environmental forensic cases. In this study, a simple sample preparation ultrahigh-performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry was established for the detection of PFASs in a wide range of water matrices. By optimizing the conditions of pretreatment and the parameters of the instrument, the developed method provided good linearity of calibration standards (R > 0.99), and demonstrated excellent MLOQ (0.008-1.2 µg/L), with spiked recoveries ranging from 57.7% to 151% for 47 targets in surface water samples, and from 45.7 to 165% for 46 targets in ground and waste water samples, respectively. This method required an injection volume of 3 µL and an analysis time of only 18 min per sample. The validation method was successfully applied to the analysis of 20 environmental water samples, in which 15 target substances with different concentrations were detected, with total concentrations of 0.082 to 262.455 μg/L. The method is simple and exclusive, and can rapidly confirm the occurrence of PFASs in different water samples, providing a convenient and fast high-throughput analysis, which is especially suitable for the application in the environmental forensic investigation of PFASs pollution.
具有不同物理化学性质的全氟和多氟烷基物质 (PFASs) 的广泛使用对环境和人类健康构成了巨大威胁。同时检测不同类别的 PFASs 是一项艰巨的任务，特别是在环境法医案件中对污染水样进行快速分析时。在这项研究中，建立了一种简单的样品制备超高效液相色谱-四极杆轨道阱高分辨率质谱法，用于检测各种水基质中的 PFASs。通过优化预处理条件和仪器参数，开发的方法为校准标准提供了良好的线性度（R > 0.99），并证明了出色的 MLOQ（0.008-1.2 µg/L），在地表水样品中 47 个目标物的加标回收率范围为 57.7%至 151%，在地下水和废水样品中 46 个目标物的加标回收率范围为 45.7%至 165%。该方法仅需 3 µL 的进样量，每个样品的分析时间仅为 18 分钟。该验证方法成功应用于 20 个环境水样的分析，其中检测到 15 种不同浓度的目标物质，总浓度为 0.082 至 262.455 µg/L。该方法简单且具有排他性，可以快速确认不同水样中 PFASs 的存在情况，提供便捷快速的高通量分析，特别适合 PFASs 污染的环境法医调查应用。
