9-甲氧基喜树碱与不同 G-四链体拓扑结构的相互作用：荧光和圆二色性研究。

Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies.

机构信息

Department of Bioanalytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.

Department of Physics and Biophysics, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 38/42, 60-637 Poznań, Poland.

出版信息

Int J Mol Sci. 2021 Sep 27;22(19):10399. doi: 10.3390/ijms221910399.

DOI:10.3390/ijms221910399

PMID:34638738

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8508660/

Abstract

The interactions of G-quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecular targets. The studied compound was selectively quenched by parallel c- G-quadruplex DNA, whereas hybrid and antiparallel G4 topology caused only a negligible decrease in the fluorescence of the ligand. A high decrease of fluorescence of the ligand after binding with G-quadruplex suggests that this molecule can be used as a selective probe for parallel G-quadruplexes.

摘要

通过荧光和圆二色光谱研究了不同拓扑结构的 G-四链体与高荧光 9-甲氧基吕林素配体 9-MeLM 的相互作用。结果表明，9-甲氧基吕林素能够相互作用且不会破坏任何被研究的分子靶标。所研究的化合物被平行 c-G-四链体 DNA 选择性猝灭，而杂交和反平行 G4 拓扑结构仅导致配体荧光的可忽略的降低。配体与 G-四链体结合后荧光的高度降低表明，该分子可作为平行 G-四链体的选择性探针。