Dröge W, Männel D, Falk W, Lehmann V, Schmidt H, Nick S, Häcker-Shahin B, Jänicke R
J Immunol. 1985 May;134(5):3379-83.
The effect of L-ornithine on several types of immune reactions was analyzed. L-ornithine was found to suppress the activation of cytotoxic T lymphocytes (CTL) in vivo and in vitro. This suppressive effect was not observed with the structural analogues D-ornithine, L-lysine, or putrescine or with the amino acids L-histidine or L-alanine. The concentration of 9 X 10(-3) M L-ornithine was found to mediate a practically complete suppression of the cytotoxic response in vitro if applied on day 0 or day 1 of the culture, but a comparably weak suppression if applied on day 3. The same concentration of L-ornithine had no effect on the production of the lymphokines interleukin 2 (IL 2) and gamma-interferon (IFN-gamma). This concentration of ornithine had also no substantial effect on several types of proliferative responses, including the allogeneic mixed lymphocyte reaction, the concanavalin A-activated IL 2-dependent proliferation of thymocytes, and IL 2-dependent proliferation of the T cell clone W-2. These observations suggest that L-ornithine inhibits selectively the differentiation of CTL effector cells. By the criteria tested, the immunosuppressive effect of L-ornithine is more selective than that of cyclosporine A, which was previously found to suppress not only the activation of cytotoxic activity but also proliferative responses and the production of the lymphokines IL 2 and IFN-gamma.
分析了L-鸟氨酸对几种免疫反应类型的影响。发现L-鸟氨酸在体内和体外均能抑制细胞毒性T淋巴细胞（CTL）的激活。其结构类似物D-鸟氨酸、L-赖氨酸、腐胺，以及氨基酸L-组氨酸或L-丙氨酸均未观察到这种抑制作用。如果在培养的第0天或第1天应用9×10⁻³M的L-鸟氨酸，发现其在体外可介导细胞毒性反应几乎完全被抑制，但在第3天应用时抑制作用相对较弱。相同浓度的L-鸟氨酸对淋巴因子白细胞介素2（IL-2）和γ-干扰素（IFN-γ）的产生没有影响。该浓度的鸟氨酸对几种增殖反应也没有实质性影响，包括同种异体混合淋巴细胞反应、伴刀豆球蛋白A激活的胸腺细胞IL-2依赖性增殖以及T细胞克隆W-2的IL-2依赖性增殖。这些观察结果表明，L-鸟氨酸选择性地抑制CTL效应细胞的分化。根据所测试的标准，L-鸟氨酸的免疫抑制作用比环孢素A更具选择性，此前发现环孢素A不仅抑制细胞毒性活性的激活，还抑制增殖反应以及淋巴因子IL-2和IFN-γ的产生。
