Teofani Adelaide, Libonati Antonio, Unida Valeria, Biocca Silvia, Desideri Alessandro, Campanella Vincenzo
Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy.
Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, Via Montpellier, 00133 Rome, Italy.
Microorganisms. 2024 Jul 24;12(8):1518. doi: 10.3390/microorganisms12081518.
Apical periodontitis is an inflammatory disease triggered by oral pathogens invading necrotic root canals. The aim of this study was to evaluate the coronal and root canal bacterial community profiles in primary endodontic infections with different periapical (PAI) indices in comparison to oral mucosa controls. A total of 31 patients with primary apical periodontitis, 14 with PAI-1 and 17 with PAI-3 were recruited. Microbial specimens from mucosa (control samples) and endodontic necrotic tissues were collected in each patient. Microbiota composition was studied through 16S ribosomal RNA gene amplicon sequencing analysis. Overall, 2953 taxa from 168 different genera of 451 various microbial species were retrieved in the controls and PAI-1 and PAI-3 groups. is the predominant phylum in the oral controls (34.5%) and PAI-1 (44.4%) groups, while is predominant in PAI-3 (38.6%). The (21.5%) and (12.5%) relative abundance is higher in oral controls while that of is higher in the PAI-1 (3.5%) and PAI-3 (2.5%) groups, being almost absent in controls (less than 0.1%). Most of the increased bacterial species found in the PAI groups were strict anaerobes. A diminished microbial diversity was found in apical periodontitis with higher PAI. These samples were also characterized by an increase in bacteria belonging to phyla and genera with an increased anaerobic character.
根尖周炎是一种由口腔病原体侵入坏死根管引发的炎症性疾病。本研究的目的是评估与口腔黏膜对照相比，不同根尖周指数（PAI）的原发性牙髓感染中冠部和根管的细菌群落概况。共招募了31例原发性根尖周炎患者，其中14例PAI为1级，17例PAI为3级。收集每位患者的黏膜微生物标本（对照样本）和牙髓坏死组织。通过16S核糖体RNA基因扩增子测序分析研究微生物群落组成。总体而言，在对照组、PAI-1组和PAI-3组中检索到来自451种不同微生物的168个不同属的2953个分类单元。 在口腔对照组（34.5%）和PAI-1组（44.4%）中是主要门类，而在PAI-3组（38.6%）中 是主要门类。 在口腔对照组中的相对丰度（21.5%）和 （12.5%）较高，而 在PAI-1组（3.5%）和PAI-3组（2.5%）中的相对丰度较高，在对照组中几乎不存在（小于0.1%）。PAI组中发现的大多数增加的细菌种类是严格厌氧菌。在PAI较高的根尖周炎中发现微生物多样性降低。这些样本的特征还在于属于具有增加的厌氧特性的门类和属的细菌增加。
