从患病养殖鱼类中分离出的O-多糖的新结构
New Structure of O-Polysaccharide Isolated from Ill Farmed Fish.
作者信息
Ucieklak Karolina, Wojtys-Tekiel Sylwia, Leroy Garance, Le Devendec Laëtitia, Baron Sandrine, Kaszowska Marta
机构信息
Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Laboratory of Marine Biotechnology and Chemistry, University of Western Brittany, EMR CNRS 6076, IUEM, 29000 Quimper, France.
出版信息
Microorganisms. 2024 Aug 1;12(8):1575. doi: 10.3390/microorganisms12081575.
The diversity of O-polysaccharides (O-antigens) among 28 strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by H and C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of O-antigen has been established: →3)-α-L-Rha-(1→3)-α-D-ManNAc-(1→2)-β-D-Glc-(1→3)-α-L-Rha2OAc4OAc-(1→3)-β-D-ManNAc-(1→3)-α-D-Glc-(1→. This new O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish.
通过使用高分辨率魔角旋转（HR MAS）核磁共振光谱法，已确定了从患病鱼类中分离出的28株菌株中O-多糖（O-抗原）的多样性。在两株分离物中鉴定出了新的O-多糖。通过氢和碳核磁共振光谱以及基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）对这种新结构进行了研究。已确定了O-抗原线性六糖重复单元的以下结构：→3)-α-L-鼠李糖-(1→3)-α-D-甘露糖胺-(1→2)-β-D-葡萄糖-(1→3)-α-L-鼠李糖2OAc4OAc-(1→3)-β-D-甘露糖胺-(1→3)-α-D-葡萄糖-(1→。这种新的O-多糖分别在2010年和2011年从鳟鱼和大菱鲆中采集的两株分离物中被检测到。应根据分离物的地理来源、鱼类种类和鱼类健康状况，对更多分离物进行进一步研究，以评估这种新O-多糖的分布情况。
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