Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland.
Carbohydr Res. 2010 Jan 26;345(2):270-4. doi: 10.1016/j.carres.2009.11.005. Epub 2009 Nov 6.
The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by (1)H, (13)C, and (31)P NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {-->4-alpha-D-Glcp-(1-->3)-alpha-L-FucpNAc-(1-->3)-[alpha-D-Glcp-(1-->4)]-alpha-D-GlcpNAc-(1-->3)-alpha-L-FucpNAc-(1-->4)-alpha-D-Glcp-(1-->P}(n).
海氏不动杆菌 PCM 1195 株的脂多糖（LPS）采用热酚/水法提取。O-特异性多糖经温和酸性水解释放，凝胶过滤分离。通过（1）H、（13）C 和（31）P NMR 光谱、MALDI-TOF MS 和 GC-MS，并结合单糖和甲基化分析，研究了 O-特异性多糖的结构。结果表明，O-特异性多糖由一个六糖重复单元组成，通过磷酸基团连接：{-->4-α-D-Glcp-(1-->3)-α-L-FucpNAc-(1-->3)-[α-D-Glcp-(1-->4)]-α-D-GlcpNAc-(1-->3)-α-L-FucpNAc-(1-->4)-α-D-Glcp-(1-->P}(n)。
