Zhang Rongtao, Wang Shenzheng, Zhong Haixiu, Fu Xiaoyu, Li Lin, Wang Li, Liu Yingnan
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Institution of Nature and Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China.
Microorganisms. 2024 Aug 12;12(8):1648. doi: 10.3390/microorganisms12081648.
Shrub invasion affects plant growth and soil physicochemical properties, resulting in soil microbiota metabolic pathway changes. However, little is known about the shrub expansion intensity of microbial metabolic pathway processes. In this study, we used metagenome sequencing technology to investigate changes in soil microbial C and N metabolic pathways and community structures, along with different shrub invasion intensities, in the Sanjiang Plain wetlands. Different shrub invasion intensities significantly affected the soil microbial composition (β diversity), with no significant effect on the α diversity compared to CK. AN, pH, and TP were the major factors influencing the microbial community's structures. Compared to CK, the shrub expansion intensity did not significantly affect C fixation and central metabolism but significantly reduced methanogenesis, which involves the CO-to-methane transition that occurs in methane metabolism, and denitrification, the nitrite to nitric oxide (nirK or nirS) transition that occurs in N metabolism. This study provides an in-depth understanding of the biogeochemical cycles of wetland ecosystems in cold northern regions undergoing shrub invasion.
灌木入侵影响植物生长和土壤理化性质，导致土壤微生物群代谢途径发生变化。然而，对于微生物代谢途径过程的灌木扩张强度知之甚少。在本研究中，我们利用宏基因组测序技术，研究了三江平原湿地不同灌木入侵强度下土壤微生物碳氮代谢途径和群落结构的变化。不同灌木入侵强度显著影响土壤微生物组成（β多样性），与对照相比，对α多样性无显著影响。全氮、pH值和总磷是影响微生物群落结构的主要因素。与对照相比，灌木扩张强度对碳固定和中心代谢没有显著影响，但显著降低了甲烷生成（涉及甲烷代谢中从一氧化碳到甲烷的转变）和反硝化作用（氮代谢中从亚硝酸盐到一氧化氮的转变，由nirK或nirS介导）。本研究深入了解了北方寒冷地区遭受灌木入侵的湿地生态系统的生物地球化学循环。
