Wang Xiangtao, Zhao Ningning, Li Wencheng, Pu Xin, Xu Peng, Wang Puchang
School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Center for Grassland Microbiome, College of Pastoral, Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China.
Microorganisms. 2024 Aug 15;12(8):1679. doi: 10.3390/microorganisms12081679.
Understanding the roles of core bacterial taxa in forage production is crucial for developing sustainable fertilization practices that enhance the soil bacteria and forage yield. This study aims to investigate the impact of different fertilization regimes on soil bacterial community structure and function, with a particular focus on the role of core bacterial taxa in contributing to soil nutrient content and enhancing forage yield. Field experiments and high-throughput sequencing techniques were used to analyze the soil bacterial community structure and function under various fertilization regimes, including six treatments, control with no amendment (CK), double the standard rate of organic manure (T01), the standard rate of organic manure with nitrogen input equal to T04 (T02), half the standard rate of inorganic fertilizer plus half the standard rate of organic manure (T03), the standard rate of inorganic fertilizer reflecting local practice (T04), and double the standard rate of inorganic fertilizer (T05). The results demonstrated that organic manure treatments, particularly T01, significantly increased the forage yield and the diversity of core bacterial taxa. Core taxa from the Actinomycetota, Alphaproteobacteria, and Gammaproteobacteria classes were crucial in enhancing the soil nutrient content, directly correlating with forage yield. Fertilization significantly influenced functions relating to carbon and nitrogen cycling, with core taxa playing central roles. The diversity of core microbiota and soil nutrient levels were key determinants of forage yield variations across treatments. These findings underscore the critical role of core bacterial taxa in agroecosystem productivity and advocate for their consideration in fertilization strategies to optimize forage yield, supporting the shift towards sustainable agricultural practices.
了解核心细菌类群在牧草生产中的作用对于制定可持续施肥措施至关重要，这些措施可增强土壤细菌并提高牧草产量。本研究旨在调查不同施肥制度对土壤细菌群落结构和功能的影响，特别关注核心细菌类群在促进土壤养分含量和提高牧草产量方面的作用。采用田间试验和高通量测序技术分析了不同施肥制度下的土壤细菌群落结构和功能，包括六种处理：不施肥对照（CK）、有机肥施用量为标准量两倍（T01）、有机肥施用量为标准量且氮肥投入量与T04相同（T02）、无机肥施用量为标准量一半加有机肥施用量为标准量一半（T03）、反映当地实际情况的无机肥标准施用量（T04）以及无机肥施用量为标准量两倍（T05）。结果表明，有机肥处理，尤其是T01，显著提高了牧草产量和核心细菌类群的多样性。放线菌门、α-变形菌纲和γ-变形菌纲的核心类群在提高土壤养分含量方面至关重要，与牧草产量直接相关。施肥显著影响了与碳氮循环相关的功能，核心类群发挥着核心作用。核心微生物群的多样性和土壤养分水平是不同处理间牧草产量变化的关键决定因素。这些发现强调了核心细菌类群在农业生态系统生产力中的关键作用，并主张在施肥策略中考虑它们以优化牧草产量，支持向可持续农业实践的转变。
