Jeong Seo Kyoung, Han Seong Eun, Vasantha-Srinivasan Prabhakaran, Jung Woo Jin, Maung Chaw Ei Htwe, Kim Kil Yong
Department of Plant Protection and Quarantine, Chonnam National University, Gwangju 61186, Republic of Korea.
Department of Agricultural Chemistry, Environmentally-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Microorganisms. 2024 Aug 16;12(8):1691. doi: 10.3390/microorganisms12081691.
Microbial agents such as the species are recognized for their role as biocontrol agents against various phytopathogens through the production of diverse bioactive compounds. This study evaluates the effectiveness of PE7 in inhibiting the growth of , the pathogen responsible for gummy stem blight (GSB) in cucurbits. Dual culture assays demonstrate significant antifungal activity of strain PE7 against . Volatile organic compounds (VOCs) produced by strain PE7 effectively impede mycelial formation in , resulting in a high inhibition rate. Light microscopy revealed that hyphae exposed to VOCs exhibited abnormal morphology, including swelling and excessive branching. Supplementing a potato dextrose agar (PDA) medium with a 30% PE7 culture filtrate significantly decreased mycelial growth. Moreover, combining a 30% culture filtrate with half the recommended concentration of a chemical fungicide yielded a more potent antifungal effect than using the full fungicide concentration alone, inducing dense mycelial formation and irregular hyphal morphology in . Strain PE7 was highly resilient and was able to survive in fungicide solutions. Additionally, PE7 enhanced the nutrient content, growth, and development of melon plants while mitigating the severity of GSB compared to fungicide and fertilizer treatments. These findings highlight PE7 as a promising biocontrol candidate for integrated disease management in crop production.
诸如该物种之类的微生物制剂因其通过产生多种生物活性化合物作为对抗各种植物病原体的生物防治剂的作用而受到认可。本研究评估了PE7对引起葫芦科作物蔓枯病（GSB）的病原菌[具体病原菌名称未给出]生长的抑制效果。对峙培养试验表明菌株PE7对[具体病原菌名称未给出]具有显著的抗真菌活性。菌株PE7产生的挥发性有机化合物（VOCs）有效阻碍了[具体病原菌名称未给出]的菌丝形成，导致高抑制率。光学显微镜显示，暴露于VOCs的[具体病原菌名称未给出]菌丝呈现出异常形态，包括肿胀和过度分支。在马铃薯葡萄糖琼脂（PDA）培养基中添加30%的PE7培养滤液显著降低了菌丝生长。此外，将30%的培养滤液与推荐浓度一半的化学杀菌剂混合，比单独使用全浓度杀菌剂产生了更强的抗真菌效果，导致[具体病原菌名称未给出]菌丝密集形成和菌丝形态不规则。菌株PE7具有高度的抗性，能够在杀菌剂溶液中存活。此外，与杀菌剂和肥料处理相比，PE7提高了甜瓜植株的养分含量、生长和发育，同时减轻了GSB的严重程度。这些发现突出了PE7作为作物生产中综合病害管理的有前途的生物防治候选物。
