Wang Guangzhao, Wang Guiping, Ma Yixiang, Lv Zhaoyun, You Yinwei, Ma Pengtao, Yu Yi
Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Shandong Institute of Sericulture, Yantai 264000, China.
Microorganisms. 2024 Aug 19;12(8):1709. doi: 10.3390/microorganisms12081709.
is an excellent pollinator in nature and plays a vital role in the conservation of agro-ecosystems and food security. Given the important role of the gut bacterial community in host health and regulation of host growth and development, using 16S rRNA gene sequencing data, the present study explored the composition of the gut bacterial community and its diversity at different life stages (eggs, young larvae, old larvae, young pupae, old pupae, and 1-day-old adults in cocoons) of the solitary bee . The results showed that the core phyla in the gut of at different life stages were Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteriota, and the core genera were , , and . The highest intestinal bacterial diversity was found in the Egg period, and the lowest bacterial alpha diversity was found in the 1-day-old Adult period; the bacterial diversity of showed a process of decreasing, as it was growing from the Egg period to the 1-day-old Adult period. Our study found that undergoes a significant change in the structure of the gut flora when it grows from the young pupae to old pupae stage, a period of growth that coincides with the process of cocooning and isolation from the external environment after food depletion. This suggests that food and environmental factors are key contributors to the structure of the bacterial community in the gut of solitary bees.
在自然界中是一种优秀的传粉者，在农业生态系统保护和粮食安全方面发挥着至关重要的作用。鉴于肠道细菌群落对宿主健康以及宿主生长发育调节的重要作用，本研究利用16S rRNA基因测序数据，探究了独居蜂在不同生命阶段（卵、幼虫初期、幼虫后期、蛹初期、蛹后期以及茧中1日龄成虫）肠道细菌群落的组成及其多样性。结果表明，独居蜂在不同生命阶段肠道中的核心菌门为变形菌门、厚壁菌门、拟杆菌门和放线菌门，核心菌属为[具体菌属1]、[具体菌属2]和[具体菌属3]。在卵期发现肠道细菌多样性最高，在1日龄成虫期发现细菌α多样性最低；随着独居蜂从卵期生长到1日龄成虫期，其细菌多样性呈现出下降的过程。我们的研究发现，独居蜂从蛹初期生长到蛹后期阶段时，肠道菌群结构发生显著变化，这一生长阶段与食物耗尽后结茧并与外部环境隔离的过程相吻合。这表明食物和环境因素是独居蜂肠道细菌群落结构的关键影响因素。
