Wu Huining, Guo Mengru, Zhao Linlu, Zhang Jin, He Jieyi, Xu Anning, Yu Zhichao, Ma Xingbin, Yong Yanhong, Li Youquan, Ju Xianghong, Liu Xiaoxi
Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524091, China.
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Pharmaceuticals (Basel). 2024 Aug 4;17(8):1023. doi: 10.3390/ph17081023.
has anti-inflammatory, antioxidant, and immune-regulating effects, while macrophages play an important role in reducing inflammation. However, it is still unclear whether extract (SGE) is effective in reducing inflammation by regulating macrophages. This study investigated the regulatory effect of SGE on macrophage polarization in a lipopolysaccharide (LPS)-induced intestinal inflammation model after establishing the model in vitro and in vivo. The results from the in vivo model showed that, compared with the LPS group, SGE significantly improved ileal morphology, restored the ileal mucosal barrier, and reduced intestinal and systemic inflammation by increasing CD206 and reducing iNOS proteins. In the in vitro model, compared with the LPS group, SGE significantly reduced the expression of iNOS protein and cytokines (TNF-α, IL-1β, and IFN-γ) while significantly increasing the protein expression of CD206 in RAW264.7 cells. In conclusion, SGE can alleviate intestinal inflammation, protect the mucus barrier, and block the systemic immunosuppressive response by increasing M2 macrophages.
具有抗炎、抗氧化和免疫调节作用，而巨噬细胞在减轻炎症方面发挥着重要作用。然而，尚不清楚提取物（SGE）是否通过调节巨噬细胞来有效减轻炎症。本研究在体外和体内建立脂多糖（LPS）诱导的肠道炎症模型后，研究了SGE对巨噬细胞极化的调节作用。体内模型的结果表明，与LPS组相比，SGE通过增加CD206和减少诱导型一氧化氮合酶（iNOS）蛋白，显著改善回肠形态，恢复回肠黏膜屏障，并减轻肠道和全身炎症。在体外模型中，与LPS组相比，SGE显著降低RAW264.7细胞中iNOS蛋白和细胞因子（肿瘤坏死因子-α、白细胞介素-1β和干扰素-γ）的表达，同时显著增加CD206的蛋白表达。总之，SGE可通过增加M2巨噬细胞来减轻肠道炎症、保护黏液屏障并阻断全身免疫抑制反应。
