Minho Alan Silva, Almeida Pamela Gomes de, Kato Natália Naomi, Brand Ana Laura Macedo, Vieira Roberto Fontes, Garrett Rafael, Lopes Norberto Peporine, Rezende Claudia Moraes, Fernandes Patricia Dias
Laboratório de Farmacologia da Dor e da Inflamação, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
NPPNS-Núcleo de Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Ciências Biomoleculares, Universidade de São Paulo, Ribeirão Preto 14040-903, Brazil.
Pharmaceuticals (Basel). 2024 Aug 22;17(8):1101. doi: 10.3390/ph17081101.
(Klatt) Lovo & A. Gil (Iridaceae) is a popularly known species with primarily ornamental economic interest. It has traditional uses as purgative, in conditions related to the menstrual cycle, for blood purification, as wound healing, and as anti-inflammatory. The anti-inflammatory and antinociceptive activities of the decoction from its aerial stems, corms, and stamens are described here with dereplication studies on LC-MS/MS supported by the GNPS platform, where phenolic compounds were annotated and correlated with its biological activity. The decoction was evaluated in chemical (formalin and capsaicin) and thermal (hot plate) induced nociception or carrageenan-induced inflammation in mice. Decoction (at 10, 30, or 100 mg/kg doses) significantly reduced formalin- or capsaicin-induced nociception. All doses also demonstrated an antinociceptive effect in the hot plate model increasing the time the animal spent in responding to thermal signal. Naloxone partially reversed the antinociceptive effect. An anti-inflammatory effect was observed since a reduction in cell migration, protein extravasation interleukin-1, and tumor necrosis factor production induced by carrageenan in the subcutaneous air pouch was quantified. Metabolomic analyses showed a predominance of phenolic substances, mainly flavonoids and chlorogenic acids. The literature showed that these two groups have significant anti-inflammatory and analgesic activity, and chemical data corroborate the pharmacological results observed.
（克拉特）洛沃鸢尾（Lovo & A. Gil，鸢尾科）是一种广为人知的物种，主要具有观赏经济价值。它在传统上被用作泻药，用于与月经周期相关的病症、血液净化、伤口愈合以及抗炎。本文描述了其地上茎、球茎和雄蕊煎剂的抗炎和抗伤害感受活性，并通过全球天然产物社会分子网络（GNPS）平台支持的液相色谱 - 串联质谱（LC-MS/MS）去重复研究，对酚类化合物进行了注释并将其与生物活性相关联。在化学（福尔马林和辣椒素）和热（热板）诱导的伤害感受或角叉菜胶诱导的小鼠炎症模型中对煎剂进行了评估。煎剂（10、30或100毫克/千克剂量）显著降低了福尔马林或辣椒素诱导的伤害感受。所有剂量在热板模型中也表现出抗伤害感受作用，增加了动物对热信号做出反应的时间。纳洛酮部分逆转了抗伤害感受作用。观察到了抗炎作用，因为对角叉菜胶在皮下气囊中诱导的细胞迁移、蛋白质外渗、白细胞介素 -1和肿瘤坏死因子产生的减少进行了量化。代谢组学分析表明酚类物质占主导，主要是黄酮类化合物和绿原酸。文献表明这两类物质具有显著的抗炎和镇痛活性，化学数据证实了所观察到的药理结果。
