Deshmukh Meghshree, Hu Zhicheng, Mohammad Majd, Jin Tao
Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 41346 Gothenburg, Sweden.
Center for Clinical Laboratories, The Affiliated Hospital of Guizhou Medical University, Guiyang 550001, China.
Pathogens. 2024 Aug 2;13(8):652. doi: 10.3390/pathogens13080652.
[] is a leading cause of sepsis and septic arthritis, conditions that pose significant medical challenges due to their high mortality and morbidity. No studies have used an in vivo imaging system [IVIS] to monitor sepsis and septic arthritis. Here, we employed a bioluminescent reporter strain of , Newman AH5016, administered intravenously to induce sepsis and intra-articularly to induce local septic arthritis in mice. Disease progression was monitored using IVIS to capture bioluminescent signals from kidneys, joints, and whole mice. Cytokines in the blood and joints were measured. The efficacy of cloxacillin treatment was evaluated. In the sepsis model, bioluminescent signals from kidneys, but not from whole mice, were correlated with kidney bacterial load and abscess formation. Ex vivo kidney imaging detected increased bacterial load and abscess formation from day 3 to day 10. Antibiotic treatment significantly reduced kidney signals, correlating with decreased bacterial counts and IL-6 levels, indicating effective infection control. In the local infection model, early-phase bioluminescent signals from joints were correlated with macroscopic arthritis and bacterial burden. Thus, signal detection from kidneys using IVIS is useful for monitoring sepsis and assessing antibiotic efficacy, though it may only be effective for early-phase monitoring of local septic arthritis.
[病原体名称]是脓毒症和脓毒性关节炎的主要病因，这些病症因其高死亡率和高发病率而带来重大医学挑战。尚无研究使用体内成像系统（IVIS）来监测脓毒症和脓毒性关节炎。在此，我们采用了一种生物发光报告菌株，即纽曼AH5016，通过静脉注射诱导脓毒症，通过关节内注射诱导小鼠局部脓毒性关节炎。使用IVIS监测疾病进展，以捕获来自肾脏、关节和整个小鼠的生物发光信号。测量血液和关节中的细胞因子。评估氯唑西林治疗的疗效。在脓毒症模型中，来自肾脏而非整个小鼠的生物发光信号与肾脏细菌载量和脓肿形成相关。离体肾脏成像检测到从第3天到第10天细菌载量增加和脓肿形成。抗生素治疗显著降低了肾脏信号，这与细菌计数和白细胞介素-6水平降低相关，表明感染得到有效控制。在局部感染模型中，来自关节的早期生物发光信号与宏观关节炎和细菌负荷相关。因此，使用IVIS从肾脏检测信号对于监测脓毒症和评估抗生素疗效是有用的，尽管它可能仅对局部脓毒性关节炎的早期监测有效。
