Weerasooriya Gayani, Dulakshi H M T, de Alwis P S, Bandara Sandun, Premarathne K R P S, Dissanayake Nayanajith, Liyanagunawardena N, Wijemuni M I, Priyantha M A R
Bacteriology Division, Veterinary Research Institute, Peradeniya P.O. Box 28, Sri Lanka.
Pathogens. 2024 Aug 7;13(8):664. doi: 10.3390/pathogens13080664.
The persistence of non-typhoidal and in chicken meat is a considerable public health risk and a future challenge. This study aimed to determine the prevalence of and in poultry processing lines where different chlorine concentrations were used in the chill tank. The samples were collected from four types of processing plants in Sri Lanka, considering the chlorine concentration used in the chill tank, which ranged from 2 ppm to 50 ppm. and were isolated from whole carcass washings, neck skin, and cecal samples. Subsequently, an antimicrobial susceptibility test was performed for the isolates. The results revealed the overall prevalence of and was 78.25% and 63.5%, respectively. Positive percentages of and were high in the carcasses compared to the neck skin and ceca. The counts on the whole carcasses were significantly low ( < 0.001), at higher chlorine concentrations ranging from 20 to 30 ppm and 40 to 50 ppm. The pathogen prevalence in the whole carcasses was 84.7% , 39.1% , 71.1% Typhimurium, and 28.8% Infantis. The highest resistance was observed for tetracycline (63.8%) in , while it was for gentamicin (87.8%) in . The prevalence percentage of multidrug-resistant was 51.2%, while it was 2.12% for . The persistence of multidrug-resistant and on the post-chill carcasses was highlighted in the present study as a significant public health threat that has to be addressed urgently.
非伤寒型沙门氏菌在鸡肉中的持续存在是一个相当大的公共卫生风险和未来挑战。本研究旨在确定在冷却池中使用不同氯浓度的家禽加工生产线中沙门氏菌的流行情况。考虑到冷却池中使用的氯浓度范围为2 ppm至50 ppm，从斯里兰卡的四种类型加工厂采集了样本。从整个胴体冲洗液、颈部皮肤和盲肠样本中分离出沙门氏菌。随后，对分离株进行了药敏试验。结果显示，沙门氏菌和肠炎沙门氏菌的总体流行率分别为78.25%和63.5%。与颈部皮肤和盲肠相比，胴体中沙门氏菌和肠炎沙门氏菌的阳性百分比更高。在氯浓度较高的20至30 ppm和40至50 ppm范围内，整个胴体上的沙门氏菌计数显著较低（P<0.001）。整个胴体中病原体的流行率为84.7%鼠伤寒沙门氏菌、39.1%肠炎沙门氏菌、71.1%鼠伤寒沙门氏菌和28.8%婴儿沙门氏菌。鼠伤寒沙门氏菌对四环素的耐药性最高（63.8%），而肠炎沙门氏菌对庆大霉素的耐药性最高（87.8%）。多重耐药鼠伤寒沙门氏菌的流行率为51.2%，而多重耐药肠炎沙门氏菌的流行率为2.12%。本研究强调了多重耐药鼠伤寒沙门氏菌和肠炎沙门氏菌在冷却后胴体上的持续存在是一个必须紧急应对的重大公共卫生威胁。
