Yu Shuang, Xu Mengmeng, Wang Zheng, Deng Yang, Xu Hockin H K, Weir Michael D, Homayounfar Negar, Fay Guadalupe Garcia, Chen Hong, Yang Deqin
Department of Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China.
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Stomatological Hospital of Chongqing Medical University, Chongqing 404100, China.
Pathogens. 2024 Aug 21;13(8):707. doi: 10.3390/pathogens13080707.
() antisense RNA (AS) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of AS on DMAHDM susceptibility and their combined impact on inhibiting biofilm formation and protecting enamel hardness. The parent UA159 and AS overexpressing (AS) were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations for planktonic bacteria (MBC-P) and biofilms (MBC-B) were measured. As the AS MBC-B was 175 μg/mL, live/dead staining, metabolic activity (MTT), colony-forming units (CFUs), biofilm biomass, polysaccharide, and lactic acid production were investigated at 175 μg/mL and 87.5 μg/mL. The MIC, MBC-P, and MBC-B values for DMAHDM for the AS strain were half those of the UA159 strain. In addition, combining AS with DMAHDM resulted in a significant 4-log CFU reduction ( < 0.05), with notable decreases in polysaccharide levels and lactic acid production. In the in vitro cariogenic model, the combination achieved the highest enamel hardness at 67.1% of sound enamel, while UA159 without DMAHDM had the lowest at 16.4% ( < 0.05). Thus, AS enhanced DMAHDM susceptibility, and their combination effectively inhibited biofilm formation and minimized enamel demineralization. The AS + DMAHDM combination shows great potential for anti-caries dental applications.
反义RNA（AS）是一种调节致龋毒力和代谢活性的非编码RNA。甲基丙烯酸二甲氨基十六烷基酯（DMAHDM）是一种用于牙科材料的甲基丙烯酸季铵盐，具有很强的抗菌活性。本研究考察了AS对DMAHDM敏感性的影响及其对抑制生物膜形成和保护牙釉质硬度的联合作用。对亲本UA159和AS过表达菌株（AS）进行了测试。测定了浮游细菌（MBC-P）和生物膜（MBC-B）的最低抑菌浓度（MIC）和最低杀菌浓度。由于AS的MBC-B为175μg/mL，因此在175μg/mL和87.5μg/mL下研究了活/死染色、代谢活性（MTT）、菌落形成单位（CFU）、生物膜生物量、多糖和乳酸产生情况。DMAHDM对AS菌株的MIC、MBC-P和MBC-B值是UA159菌株的一半。此外，AS与DMAHDM联合使用可使CFU显著减少4个对数（<0.05），多糖水平和乳酸产生显著降低。在体外致龋模型中，该组合使牙釉质硬度达到完好牙釉质的67.1%，为最高，而未使用DMAHDM的UA159组最低，为16.4%（<0.05）。因此，AS增强了对DMAHDM的敏感性，它们的组合有效地抑制了生物膜的形成，并将牙釉质脱矿降至最低。AS+DMAHDM组合在抗龋牙科应用中显示出巨大潜力。
