Stasiak-Różańska Lidia, Berthold-Pluta Anna, Aleksandrzak-Piekarczyk Tamara, Koryszewska-Bagińska Anna, Garbowska Monika
Department of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska St. 166, 02-787 Warsaw, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
Polymers (Basel). 2024 Aug 16;16(16):2316. doi: 10.3390/polym16162316.
Bacterial cellulose (BC) is a biodegradable polymer resembling paper after being dried. It finds a growing number of applications in many branches of industry and in medicine. In the present study, BC was produced after ATCC 23769 strain culture and used as a matrix for plant extracts (tulsi, brahmi, lemon, blackberry, nettle root, and nettle leave) and essential oils (cinnamon, sage, clove, mint, thyme, lemongrass, rosemary, lemon, anise, tea tree, lime, grapefruit, and tangerine), and the antimicrobial properties of these biomaterials was determined. The growth-inhibiting effects of plant extracts and essential oils combined with BC were analyzed against five species isolated from food matrix and two reference strains from the ATCC (513229 and 29544). Additional analyses were conducted for BC water activity and for its capability to absorb biologically active plant compounds. The cellulose matrix with a 50% extract from brahmi was found to effectively inhibit the growth of the selected strains. The other plant water extracts did not show any antimicrobial activity against the tested strains. It was demonstrated that BC soaked with thyme essential oil was characterized with the strongest antimicrobial activity in comparison to the other tested EOs. These study results indicate the feasibility of deploying BC impregnated with natural plant components as an active and environmentally-friendly packaging material.
细菌纤维素（BC）是一种可生物降解的聚合物，干燥后类似纸张。它在许多工业分支和医学领域的应用越来越多。在本研究中，通过ATCC 23769菌株培养生产出BC，并将其用作植物提取物（罗勒、婆罗门参、柠檬、黑莓、荨麻根和荨麻叶）和精油（肉桂、鼠尾草、丁香、薄荷、百里香、柠檬草、迷迭香、柠檬、茴芹、茶树、酸橙、葡萄柚和橘子）的基质，然后测定这些生物材料的抗菌性能。分析了植物提取物和精油与BC结合对从食品基质中分离出的5种菌株以及ATCC的两种参考菌株（513229和29544）的生长抑制作用。还对BC的水分活度及其吸收生物活性植物化合物的能力进行了额外分析。发现含有50%婆罗门参提取物的纤维素基质能有效抑制所选菌株的生长。其他植物水提取物对受试菌株未显示出任何抗菌活性。结果表明，与其他测试的精油相比，用百里香精油浸泡的BC具有最强的抗菌活性。这些研究结果表明，将浸渍有天然植物成分的BC用作活性和环保包装材料是可行的。
