Lan Genqian, Wu Mingzhao, Zhang Qihang, Yuan Bo, Shi Guangxin, Zhu Ni, Zheng Yibingyue, Cao Qiang, Qiao Qin, Zhang Ticao
School of Agriculture, Yunnan University, Kunming 650091, China.
College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China.
Plants (Basel). 2024 Aug 13;13(16):2241. doi: 10.3390/plants13162241.
Strawberries are mainly propagated by stolons, which can be divided into monopodial and sympodial types. Monopodial stolons consistently produce ramets at each node following the initial single dormant bud, whereas sympodial stolons develop a dormant bud before each ramet. Sympodial stolon encompasses both dormant buds and ramet buds, making it suitable for studying the formation mechanism of different stolon types. In this study, we utilized sympodial stolons from as materials and explored the mechanisms underlying sympodial stolon development through transcriptomic and phytohormonal analyses. The transcriptome results unveiled that auxin, cytokinin, and sugars likely act as main regulators. Endogenous hormone analysis revealed that the inactivation of auxin could influence bud dormancy. Exogenous cytokinin application primarily induced dormant buds to develop into secondary stolons, with the proportion of ramet formation being very low, less than 10%. Furthermore, weighted gene co-expression network analysis identified key genes involved in ramet formation, including auxin transport and response genes, the cytokinin activation gene , and glucose transport genes and . Consistently, in vitro cultivation experiments confirmed that glucose enhances the transition of dormant buds into ramets within two days. Collectively, cytokinin and glucose act as dormant breakers, with cytokinin mainly driving secondary stolon formation and glucose promoting ramet generation. This study improved our understanding of stolon patterning and bud development in the sympodial stolon of strawberries.
草莓主要通过匍匐茎繁殖，匍匐茎可分为单轴型和合轴型。单轴匍匐茎在最初的单个休眠芽之后，每个节上持续产生分株，而合轴匍匐茎在每个分株之前发育出一个休眠芽。合轴匍匐茎包含休眠芽和分株芽，使其适合研究不同匍匐茎类型的形成机制。在本研究中，我们利用[未提及品种]的合轴匍匐茎作为材料，通过转录组学和植物激素分析探索合轴匍匐茎发育的潜在机制。转录组结果表明，生长素、细胞分裂素和糖类可能是主要调节因子。内源激素分析显示，生长素的失活可能影响芽的休眠。外源施用细胞分裂素主要诱导休眠芽发育成次生匍匐茎，分株形成的比例非常低，不到10%。此外，加权基因共表达网络分析确定了参与分株形成的关键基因，包括生长素运输和响应基因、细胞分裂素激活基因[未提及具体基因名称]以及葡萄糖运输基因[未提及具体基因名称]和[未提及具体基因名称]。同样，体外培养实验证实，葡萄糖在两天内增强了休眠芽向分株的转变。总体而言，细胞分裂素和葡萄糖起到打破休眠的作用，细胞分裂素主要促进次生匍匐茎形成，而葡萄糖促进分株产生。本研究增进了我们对草莓合轴匍匐茎中匍匐茎模式和芽发育的理解。
