Kupriyanova Elena V, Sinetova Maria A, Gabrielyan David A, Los Dmitry A
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, Russia.
Plants (Basel). 2024 Aug 20;13(16):2323. doi: 10.3390/plants13162323.
Under standard laboratory conditions, PCC 7942 lacks EcaA, a periplasmic carbonic anhydrase (CA). In this study, a transformant was created that expressed the homologous EcaA from sp. ATCC 51142. This additional external CA had no discernible effect on the adaptive responses and physiology of cells exposed to changes similar to those found in natural habitats, such as fluctuating CO and HCO concentrations and ratios, oxidative or light stress, and high CO. The transformant had a disadvantage over wild-type cells under certain conditions (Na depletion, a reduction in CO). cells lacked their own EcaA in all experimental conditions. The results suggest the presence in of mechanisms that limit the appearance of EcaA in the periplasm. For the first time, we offer data on the expression pattern of CCM-associated genes during adaptation to CO replacement with HCO, as well as cell transfer to high CO levels (up to 100%). An increase in CO concentration coincides with the suppression of the NDH-1 system, which was previously thought to function constitutively.
在标准实验室条件下，集胞藻PCC 7942缺乏周质碳酸酐酶（CA）EcaA。在本研究中，构建了一个表达来自集胞藻sp. ATCC 51142同源EcaA的转化体。这种额外的胞外CA对暴露于类似于自然栖息地中所发现变化（如波动的CO和HCO浓度及比例、氧化或光胁迫以及高CO）的细胞的适应性反应和生理没有明显影响。在某些条件下（Na耗尽、CO降低），该转化体相对于野生型细胞具有劣势。在所有实验条件下，细胞都缺乏自身的EcaA。结果表明集胞藻中存在限制EcaA在周质中出现的机制。我们首次提供了关于在适应用HCO替代CO以及细胞转移到高CO水平（高达100%）过程中CCM相关基因表达模式的数据。CO浓度的增加与之前认为组成型发挥作用NDH - 1系统的抑制相吻合。
