Cao Xing, Ding Liping, Liang Jiahui, Zhou Yanrong, Chen Xiulan, Li Haiyan, Liu Tao, Yue Wenxiu, Sui Juanjuan, Jiang Liangbao, Qian Yulian, Yang Dongdong, Wang Bo, Zhang Hailing, Wu Ze, Song Xiaoyan
College of Architecture, Yantai University, Yantai 264005, China.
Key Laboratory of Landscaping Agriculture, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Plants (Basel). 2024 Aug 21;13(16):2330. doi: 10.3390/plants13162330.
In our previous research, we found that trichokonins' (TKs) employment improved the thermotolerance of the Lanzhou lily, a renowned edible crop species endemic to China that is relatively susceptible to high temperatures (HTs). Here, a novel Lanzhou lily GRAS gene, , was identified to respond to heat stress (HS) and HS+TKs treatment based on transcriptome and RT-qPCR analysis. TKs could improve the upregulation of during long-term HS. The expression profile of in response to HS with or without TKs treatment showed a significant positive correlation with , which was previously identified as a key regulator in TKs' conferred resilience to HT. More importantly, overexpression of in the lily enhanced its tolerance to HTs and silencing in the lily reduced heat resistance. Taken together, this study identified the positive role of LzSCL9 in TK-induced thermotolerance, thereby preliminarily establishing a molecular mechanism on TKs regulating the thermostability of the Lanzhou lily and providing a new candidate regulator for plant heat-resistant breeding.
在我们之前的研究中，我们发现使用木霉菌毒素（TKs）可提高兰州百合的耐热性。兰州百合是中国特有的著名食用作物品种，相对易受高温影响。在此，基于转录组和RT-qPCR分析，鉴定出一个新的兰州百合GRAS基因，其对热胁迫（HS）和HS + TKs处理有响应。在长期热胁迫期间，TKs可促进该基因的上调表达。在有或无TKs处理的情况下，该基因对热胁迫的表达谱与先前被确定为TKs赋予耐热性关键调节因子的基因显著正相关。更重要的是，百合中该基因的过表达增强了其对高温的耐受性，而百合中该基因的沉默则降低了耐热性。综上所述，本研究确定了LzSCL9在TK诱导的耐热性中的积极作用，从而初步建立了TKs调节兰州百合热稳定性的分子机制，并为植物耐热育种提供了一个新的候选调节因子。
