Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Mathematical and Economic Modelling (MAEMOD), Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Pharm Biol. 2024 Dec;62(1):666-675. doi: 10.1080/13880209.2024.2395517. Epub 2024 Aug 28.
Plant peptides garner attention for their potential antimicrobial properties amid the rising concern over antibiotic-resistant bacteria.
This study investigates the antibacterial potential of crude peptide extracts from 27 Thai plants collected locally.
Peptide extracts from 34 plant parts, derived from 27 Thai plants, were tested for their antimicrobial efficacy against four highly resistant bacterial strains: MRSA, , , and . The stability of these peptide extracts was examined at different temperatures, and the synergistic effects of two selected plant peptide extracts were investigated. Additionally, the time-kill kinetics of the individual extracts and their combination were determined against the tested pathogens.
Peptides from L. and O. Fedtsch (Amaryllidaceae) were particularly potent, inhibiting bacterial growth with MICs ranging from 1.43 to 86.50 µg/mL. The consistent MICs and MBCs of these extracts across various extraction time points highlight their reliability. Stability tests reveal that these peptides maintain their antimicrobial activity at -20 °C for over a month, emphasizing their durability for future exploration and potential applications in addressing antibiotic resistance. Time-kill assays elucidate the time and concentration-dependent nature of these antimicrobial effects, underscoring their potent initial activity and sustained efficacy over time.
This study highlights the antimicrobial potential of -derived peptides, endorsing them for combating antibiotic resistance and prompting further investigation into their mechanisms.
由于对抗生素耐药菌的担忧不断增加，植物肽因其潜在的抗菌特性而受到关注。
本研究调查了从当地采集的 27 种泰国植物的粗肽提取物的抗菌潜力。
测试了来自 27 种泰国植物的 34 个植物部位的肽提取物对四种高度耐药的细菌菌株（MRSA、、、和）的抗菌功效。研究了这些肽提取物在不同温度下的稳定性，并研究了两种选定的植物肽提取物的协同作用。此外，还测定了单独提取物及其组合对测试病原体的时间杀伤动力学。
来自 石蒜科的 和 的肽特别有效，其 MIC 范围为 1.43 至 86.50µg/mL，抑制细菌生长。这些提取物在不同提取时间点的一致 MIC 和 MBC 突出了它们的可靠性。稳定性测试表明，这些肽在-20°C 下可保持一个月以上的抗菌活性，强调了它们在未来探索和解决抗生素耐药性方面的潜在应用的耐用性。时间杀伤试验阐明了这些抗菌作用的时间和浓度依赖性，突出了它们最初的强大活性和随着时间的推移的持续疗效。
本研究强调了 -衍生肽的抗菌潜力，支持将其用于对抗抗生素耐药性，并促使进一步研究其机制。
