Chen Xiaoqiong, Guo Qiaoling, Yang Xiaoli, Yuan Meng, Song Jianguo, Fu Hongyan, Zhang Hongyu, Xu Peizhou, Liao Yongxiang, Ali Asif, Du Kangxi, Wu Xianjun
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, China.
Front Plant Sci. 2024 Aug 14;15:1452520. doi: 10.3389/fpls.2024.1452520. eCollection 2024.
Previous studies have modified rice's resistant starch (RS) content by mutating single and double genes. These mutations include knocking out or reducing the expression of or genes, as well as overexpressing . However, the impact of triple mutant //OE- on RS contents remained unknown. Here, we constructed a double mutant with /RNAi-, based on with , and a triple mutant with /RNAi-/OE- , based on the double mutant. The results showed that the amylose and RS contents gradually increased with an increase in the number of mutated genes. The triple mutant exhibited the highest amylose and RS contents, with 41.92% and 4.63%, respectively, which were 2- and 5-fold higher than those of the wild type, which had 22.19% and 0.86%, respectively. All three mutants altered chain length and starch composition compared to the wild type. However, there was minimal difference observed among the mutants. The gene contributed to the improvement of 1000-grain weight and seed-setting rate, in addition to the highest amylose and RS contents. Thus, our study offers valuable insight for breeding rice cultivars with a higher RS content and yields.
先前的研究通过对单基因和双基因进行突变来改变水稻的抗性淀粉（RS）含量。这些突变包括敲除或降低 或 基因的表达，以及过表达 。然而，三突变体//OE- 对RS含量的影响仍然未知。在此，我们基于 与 构建了一个 /RNAi- 的双突变体，以及基于该双突变体构建了一个 /RNAi-/OE- 的三突变体。结果表明，直链淀粉和RS含量随着突变基因数量的增加而逐渐增加。三突变体的直链淀粉和RS含量最高，分别为41.92%和4.63%，分别是野生型（野生型直链淀粉和RS含量分别为22.19%和0.86%）的2倍和5倍。与野生型相比，所有三个突变体均改变了链长和淀粉组成。然而，突变体之间观察到的差异很小。除了具有最高的直链淀粉和RS含量外， 基因还有助于提高千粒重和结实率。因此，我们的研究为培育具有较高RS含量和产量的水稻品种提供了有价值的见解。
