Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Frontiers Science Center for Synthetic Biology (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
J Agric Food Chem. 2024 Sep 11;72(36):19566-19580. doi: 10.1021/acs.jafc.4c05028. Epub 2024 Aug 29.
As high-performance monomers for the manufacture of polyamide materials, mid- and long-chain dicarboxylic acids (DCA, ≥ 6) have received extensive attention from researchers. Biosynthesis is gradually replacing chemical synthesis due to its outstanding advantages in the industrial production of mid- and long-chain dicarboxylic acids, which is mostly achieved by using the strong terminal oxidation ability of nonmodel microorganisms such as to oxidize hydrophobic substrates such as alkanes. Here, we first summarize the metabolic pathways of oxidative alkane conversion into dicarboxylic acid by terminally oxidizing unconventional yeasts and the corresponding metabolic engineering strategies. Then, we summarize the research progress on new dicarboxylic acid production processes. Finally, the future development directions in the biosynthesis of mid- and long-chain dicarboxylic acids are prospected from synthetic biology and bioprocess engineering, which can also provide a reference for the synthesis of other biobased chemicals and biomaterials.
作为制造聚酰胺材料的高性能单体，中长链二元羧酸（DCA，≥6）受到了研究人员的广泛关注。由于其在中长链二元羧酸工业生产中的突出优势，生物合成正逐渐取代化学合成，这主要是通过利用非模式微生物如 等的强末端氧化能力来氧化疏水性底物如烷烃来实现的。在这里，我们首先总结了非常规酵母通过末端氧化将烷烃转化为二酸的代谢途径，以及相应的代谢工程策略。然后，我们总结了新的二酸生产工艺的研究进展。最后，从合成生物学和生物过程工程的角度展望了中长链二元羧酸生物合成的未来发展方向，这也可为其他生物基化学品和生物材料的合成提供参考。
