Sirchia G, Zanella A, Parravicini A, Morelati F, Rebulla P, Masera G
Transfusion. 1985 Mar-Apr;25(2):110-2. doi: 10.1046/j.1537-2995.1985.25285169198.x.
Clinical and serological data on 1435 Italian thalassemia major patients were collected during a cooperative study involving 19 centers in 10 regions. The main findings were as follows: 18 percent of the patients were under 6 years of age, 63 percent between 6 and 15, and 19 percent over 15. Forty-one percent had undergone splenectomy. Sixty-two percent of the patients were maintained at pretransfusion hemoglobin levels higher than 10 g per dl, 36 percent between 8 and 10 g per dl, and 2 percent below 8 g per dl. Overall, 5.2 percent of the patients had clinically significant red cell alloantibodies (136 alloantibodies in 74 patients). One-half of the immunized patients had more than one and one-fourth had more than two alloantibodies. The specificities of the 136 alloantibodies were almost exclusively confined to the common antigens of the Rh, Kell, Kidd, and Duffy systems, in that decreasing order of frequency. The antibody screening procedure, using a low-ionic-strength solution antiglobulin test against a three-red-cell panel and the patient's own red cells (autocontrol) with a serum to cell ratio of 100 to 1 was shown to be an adequate technique for red cell antibody detection.
在一项涉及意大利10个地区19个中心的合作研究中，收集了1435例重型地中海贫血患者的临床和血清学数据。主要研究结果如下：18%的患者年龄在6岁以下，63%的患者年龄在6至15岁之间，19%的患者年龄超过15岁。41%的患者接受过脾切除术。62%的患者输血前血红蛋白水平维持在高于每分升10克，36%的患者在每分升8至10克之间，2%的患者低于每分升8克。总体而言，5.2%的患者有临床上显著的红细胞同种抗体（74例患者中有136种同种抗体）。一半的免疫患者有不止一种同种抗体，四分之一的患者有不止两种同种抗体。136种同种抗体的特异性几乎完全局限于Rh、Kell、Kidd和Duffy系统的常见抗原，按频率递减顺序排列。使用低离子强度溶液抗球蛋白试验检测针对一个三红细胞面板和患者自身红细胞（自身对照）的抗体，血清与细胞比例为100比1，该抗体筛查程序被证明是一种检测红细胞抗体的合适技术。
