Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Bobrzynskiego 14, 30-348 Krakow, Poland; Jagiellonian University, Doctoral School of Exact and Natural Sciences, Prof. St. Łojasiewicza 11, 30-348 Krakow, Poland.
Jagiellonian University, Faculty of Chemistry, Gronostajowa 2, 30-387 Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jan 5;324:124995. doi: 10.1016/j.saa.2024.124995. Epub 2024 Aug 17.
Raman Optical Activity combined with Circularly Polarized Luminescence (ROA-CPL) was used in the spectral recognition of glutathione peptide (GSH) and its model post-translational modifications (PTMs). We demonstrate the potential of ROA spectroscopy and CPL probes (EuCl, Na[Eu(DPA)], NaEuEDTA) in the study of unmodified peptide, i.e. GSH, and its derivatives, i.e. glutathione oxidized (GSSG), S-acetylglutathione (GSAc) and S-nitrosoglutathione (GSNO). ROA spectral features of GSH, GSSG, and GSAc were determined along with thier changes upon the different pH conditions. Apart from the ROA, induced CPL signals of Eu(III) probes also proved to be sensitive to the structural modifications of GSH-based model PTMs, enabling their spectral recognition, especially by the NaEuEDTA probe.
拉曼旋光活性与圆二色性荧光（ROA-CPL）结合，用于谷胱甘肽肽（GSH）及其模型翻译后修饰（PTM）的光谱识别。我们展示了 ROA 光谱学和 CPL 探针（EuCl、Na[Eu（DPA）]、NaEuEDTA）在未修饰肽（即 GSH）及其衍生物（即氧化谷胱甘肽（GSSG）、S-乙酰谷胱甘肽（GSAc）和 S-亚硝基谷胱甘肽（GSNO））研究中的潜力。确定了 GSH、GSSG 和 GSAc 的 ROA 光谱特征，以及它们在不同 pH 值条件下的变化。除了 ROA，Eu（III）探针的诱导 CPL 信号也被证明对 GSH 基模型 PTM 的结构修饰敏感，能够对其进行光谱识别，特别是通过 NaEuEDTA 探针。
