Hillensjö T, Sjögren A, Strander B, Andino N
Acta Endocrinol (Copenh). 1985 Mar;108(3):407-13. doi: 10.1530/acta.0.1080407.
The secretion of progesterone, testosterone, and oestradiol by intact human oocyte-cumulus complexes in vitro was examined in incubations lasting 6-24 h. The complexes were aspirated from preovulatory follicles in 32 women who, due to tubal disease, were participating in an in vitro fertilization program. In 12 of the women follicular maturation was induced with clomiphene and human chorionic gonadotrophin (hCG), in 13 women with human menopausal gonadotrophin (hMG) and hCG and in 7 women with a combination of clomiphene-hMG plus hCG. The net secretion of steroids into the fertilization medium was studied before (0-6 h) and after (6-24 h) the addition of sperm, by RIA of aliquots removed at specific times. A high and sustained secretion of progesterone was found both before and after insemination. Testosterone secretion remained at a low and constant level while a net release of oestradiol was found mainly during the first hours of incubation. The release of steroids, particularly progesterone, varied according to the mode of hormonal stimulation in vivo and was highest in complexes from clomiphene-hMG-treated women, probably reflecting different maturity of the aspirated follicles. In a second series of experiments the dispersed cumulus cells were recovered after fertilization and cultured as monolayers for 2-4 days. The cells underwent spontaneous luteinization and secreted high amounts of progesterone. These results extend previous work in animals showing that also in the human the periovulatory cumulus cells are steroidogenically active. The results also suggest a functional difference in the cumulus cells related to the mode of ovulation induction.
对完整的人卵母细胞 - 卵丘复合体在体外6 - 24小时孵育期间孕酮、睾酮和雌二醇的分泌情况进行了检测。这些复合体是从32名因输卵管疾病参与体外受精项目的女性的排卵前卵泡中抽吸出来的。其中12名女性用克罗米芬和人绒毛膜促性腺激素（hCG）诱导卵泡成熟，13名女性用人绝经期促性腺激素（hMG）和hCG诱导卵泡成熟，7名女性用克罗米芬 - hMG加hCG联合诱导卵泡成熟。通过放射免疫分析法（RIA）检测特定时间取出的等分试样，研究了添加精子前后（0 - 6小时和6 - 24小时）类固醇向受精培养基中的净分泌情况。授精前后均发现孕酮有高且持续的分泌。睾酮分泌维持在低且恒定的水平，而雌二醇的净释放主要发生在孵育的最初几个小时。类固醇的释放，尤其是孕酮的释放，因体内激素刺激方式而异，在接受克罗米芬 - hMG治疗的女性的复合体中最高，这可能反映了抽吸卵泡的不同成熟度。在第二系列实验中，受精后回收分散的卵丘细胞并单层培养2 - 4天。这些细胞发生自发黄体化并分泌大量孕酮。这些结果扩展了之前在动物身上的研究工作，表明在人类中排卵周围的卵丘细胞同样具有类固醇生成活性。结果还表明卵丘细胞在与排卵诱导方式相关方面存在功能差异。
