Botero-Ruiz W, Laufer N, DeCherney A H, Polan M L, Haseltine F P, Behrman H R
Fertil Steril. 1984 Jun;41(6):820-6. doi: 10.1016/s0015-0282(16)47892-1.
Follicular fluids (FF) and their matched oocytes were obtained from 64 follicles of 28 women who failed to conceive after in vitro fertilization ( IVF ) and 33 follicles of 8 women who successfully conceived after the procedure. Ovulation was induced with human menopausal gonadotropin, and follicular aspiration was performed 36 hours after human chorionic gonadotropin administration. The concentration of 17 beta-estradiol, progesterone, testosterone, dihydrotestosterone, and androsterone was correlated with the morphology of the oocyte-corona-cumulus complex ( OCCC ), oocyte fertilization, the rate of cleavage, and the incidence of pregnancy after embryo transfer. In both groups of women, FF progesterone was lowest in follicles containing immature OCCCs . However, follicles aspirated from women who conceived after IVF which contained intermediate and mature OCCCs had significantly higher FF estradiol levels than similar follicles from women who failed to conceive after the procedure. Fertilized oocytes and 4- to 6-cell stage embryos which were obtained from follicles of pregnant women contained significantly higher FF estradiol levels than fertilized oocytes and similar embryos from nonpregnant women. It appears that higher FF estradiol levels correlate well with successful fertilization and an enhanced cleavage rate of oocytes associated with pregnancy following IVF .
从28名体外受精（IVF）后未受孕女性的64个卵泡以及8名该手术后成功受孕女性的33个卵泡中获取卵泡液（FF）及其匹配的卵母细胞。用人绝经期促性腺激素诱导排卵，并在注射人绒毛膜促性腺激素36小时后进行卵泡抽吸。17β-雌二醇、孕酮、睾酮、二氢睾酮和雄酮的浓度与卵母细胞-放射冠-卵丘复合体（OCCC）的形态、卵母细胞受精、分裂率以及胚胎移植后的妊娠发生率相关。在两组女性中，含有未成熟OCCC的卵泡中FF孕酮最低。然而，从IVF后受孕女性抽吸的含有中等和成熟OCCC的卵泡，其FF雌二醇水平明显高于该手术后未受孕女性的类似卵泡。从孕妇卵泡中获得的受精卵和4至6细胞期胚胎，其FF雌二醇水平明显高于未孕女性的受精卵和类似胚胎。看来，较高的FF雌二醇水平与IVF后与妊娠相关的成功受精和卵母细胞分裂率提高密切相关。
