Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Ecotoxicol Environ Saf. 2024 Oct 1;284:116956. doi: 10.1016/j.ecoenv.2024.116956. Epub 2024 Aug 28.
Grass carp hemorrhagic disease is caused by the grass carp reovirus (GCRV). The disease spreads rapidly and has a high fatality rate, which seriously affects grass carp culture. Moreover, the molecular mechanisms underlying grass carp hemorrhagic disease remain unclear. To decipher the effects of GCRV on grass carp tissues, resistant grass carp A (GA) and susceptible grass carp B (GB) were selected through GCRV treatment, and control grass carp C (GC) was also established. The gill, liver, and muscle tissues exhibited different onset symptoms under the influence of GCRV by histological observation. We selected muscle samples with significant differences in symptoms for Illumina RNA sequencing. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes revealed 3512, 3074, and 1853 differentially expressed genes between "GC vs. GB," "GC vs. GA," and "GA vs. GB," respectively. Additionally, 40 differential immune-related genes and 28 differential interferon-stimulating genes (ISGs) related to the interferon (IFN) pathway were identified. The expression of immunogene-related genes of GB and GA, such as MDA5, IL-34, NF-KB, TRIM25, SOCS3, CEBPB, and BCL2, and genes associated with the JAK-STAT signaling pathway, such as IRF4, STAT1, STAT3, JAK 1, and JAK 2, was significantly upregulated. The IFN and JAK-STAT signaling pathways were closely related to anti-GCRV infection. The transcriptome data and predicted immune genes and ISGs in this study provide novel insights into the treatment of GCRV.
草鱼出血病是由草鱼出血病病毒（GCRV）引起的。这种疾病传播迅速，死亡率高，严重影响草鱼养殖。此外，草鱼出血病的分子机制尚不清楚。为了解析 GCRV 对草鱼组织的影响，通过 GCRV 处理选择了抗草鱼 A（GA）和易感草鱼 B（GB），并建立了对照草鱼 C（GC）。通过组织学观察，发现草鱼的鳃、肝和肌肉组织在 GCRV 的影响下出现不同的发病症状。我们选择了症状差异明显的肌肉样本进行 Illumina RNA 测序。通过基因本体论和京都基因与基因组百科全书的分析，分别在“GC 与 GB”、“GC 与 GA”和“GA 与 GB”之间鉴定到 3512、3074 和 1853 个差异表达基因。此外，还鉴定到 40 个差异免疫相关基因和 28 个与干扰素（IFN）途径相关的差异干扰素刺激基因（ISGs）。GB 和 GA 的免疫基因相关基因，如 MDA5、IL-34、NF-KB、TRIM25、SOCS3、CEBPB 和 BCL2，以及与 JAK-STAT 信号通路相关的基因，如 IRF4、STAT1、STAT3、JAK1 和 JAK2 的表达显著上调。IFN 和 JAK-STAT 信号通路与抗 GCRV 感染密切相关。本研究中的转录组数据和预测的免疫基因和 ISGs 为 GCRV 的治疗提供了新的见解。
